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Plasmid#59132PurposeAn AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.DepositorInsertNuclear-localized Infrared Fluorescent Protein
UseAAVTags3xNLSExpressionMammalianMutationPromotermFosAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI
Plasmid#126477PurposeExpresses Cas9-5xFlag-TdT in mammalian cells.DepositorInsertsUseTagsExpressionMammalianMutationChanged codons to reduce repetitive sequences.PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti TMEM30A
Plasmid#171789Purposetransfer plasmid for lentiviral vector production expressing TMEM30ADepositorInsertTMEM30A (TMEM30A Human)
UseLentiviralTags6X His + FlagExpressionMutationPromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q
Plasmid#184249PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationCodon optimization for protein expression in BL2…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human 3' HP1a AID GFP PuroR
Plasmid#127906PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a GeneDepositorInsertHP1 (CBX5 Human, Mustard Weed)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' Hp1aPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mScarlet-gamma9
Plasmid#140097PurposeExpresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocationDepositorInsertGNGT2
UseTagsN-terminal mScarlet tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7B2Jnk3a2
Plasmid#19729DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G
Plasmid#166011Purposemammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)DepositorInsertSARS-CoV-2 S (spike) (S )
UseTags3C-His8-Strep2x2ExpressionMammalianMutationD614GPromoterCMV51Available sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSc1-DD
Plasmid#80439PurposeExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsE. coli dihydrofolate reductaseExpressionMammalianMutationPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGE3.0
Plasmid#165988PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CoV2-Omicron BA.1 spike
Plasmid#194602PurposeMammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)DepositorInsertCoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP
Plasmid#47904PurposeAn AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.DepositorInserteNpHR3.0
UseAAVTagsiRFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP
Plasmid#137139PurposeIntersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-ChR2(ET/TC)-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE123T, T159CPromoternEFAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
L40C-CRISPR.EFS.mNeon
Plasmid#69146PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
xCas12i
Plasmid#195333Purposevector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized xCas12i
UseTags3xFlagExpressionMammalianMutationPromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-117G-hyeA3A-BE4max
Plasmid#157946PurposeLentiviral expression of hyeA3A-BE4maxDepositorInsertLenti -117G-hyeA3A-BE4max
UseCRISPR and LentiviralTagsExpressionMammalianMutationN57GPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS
Plasmid#128833PurposeKnock-in of nuclear EYFP into mouse Elf5 locusDepositorInsertNuclear EYFP into Elf5 locus (Elf5 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆cap
Plasmid#74786Purposemammalian expression of the capping activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsExpressionMammalianMutationV689D, L693DPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)
Plasmid#100944PurposeSingle chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tagDepositorInsertscFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)
UseTags3xTy1ExpressionMammalianMutationF636A, E660A, E725A, R295APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSCRET2(#323)
Plasmid#184058Purposecyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesisDepositorInsert6myc-CRE-ERT2
UseSynthetic BiologyTags6xMycExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only