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Plasmid#87770PurposeExpresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)DepositorInsertMKP5 (DUSP10 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-DN-REST
Plasmid#61533PurposeExpresses DN-REST in mammalian cellsDepositorInsertREST (REST Human)
UseLentiviralTagsExpressionMammalianMutationP73, Dominant negative REST (contains the Zf DNA-…PromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Blast-H2BC11
Plasmid#207757PurposeDonor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-EF1a-Tmx4-V5
Plasmid#120236PurposeExpresses V5-tagged Tmx4 in lentiviral vectorDepositorInsertTmx4 (Tmx4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mNeon-Blast-TUBB4B
Plasmid#207786PurposeDonor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785DepositorArticleInsertTUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CMV/TO_PRKAA1
Plasmid#74446PurposeLentiviral expression vector encoding untagged WT human AMPK alpha 1DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRGEB-Cas9-VirD2 (PCO)
Plasmid#139802PurposeExpressing Cas9-VirD fusion under ubiquitin promoterDepositorInsertCas9-VirD2
UseTagsCas9-VirD2ExpressionPlantMutationPromoterUbiquitinAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pENTR1A DHFR.cHSF1
Plasmid#134737PurposeGateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseEntry vector for gateway cloningTagsDHFRExpressionMutationDeletion of amino acids 186-202PromoterNoneAvailable sinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE
Plasmid#82718PurposeExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.DepositorInsertMKK6 (MAP2K6 Human)
UseTagsExpressionBacterialMutationSer207 to Glu, Thr211 to GluPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHalo-MyosinIIB-C25
Plasmid#128574PurposeStudies of cellular contractility.DepositorInsertMyosin IIB (MYH10 Human)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68
Plasmid#126548PurposeExpresses FLAG-HA-tagged RNF68 in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationPromoterLTRAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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AA290
Plasmid#215949PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertU6_v1; sgCD55_v4; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA286
Plasmid#215948PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertU6_v1; sgCD47_v6; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLC-EGFP
Plasmid#193566PurposeExpresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
AA031
Plasmid#216011PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorInsertnCas9-SpRY (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_936
Plasmid#216094PurposeCas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive controlDepositorInsertCD46, CD47, CD55, CD81 guides
UseCRISPR and Lentiviral; Positive controlsTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRV FOXP3 WWRR
Plasmid#13625DepositorInsertFOXP3 (FOXP3 Human)
UseRetroviralTagsMycExpressionMammalianMutationWWRR: T359W, N361W, E399R, E401RPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only