Addgene

We narrowed to 18,521 results for: omp

Showing: 8981 - 9000 of 18521 results

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

Plasmid

#172581

Purpose

For transient expression of C-terminal BirA-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

BirA(R118G)-HA

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A321G

Plasmid

#98670

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A321G

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

Plasmid

#75133

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

Plasmid

#127363

Purpose

Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPERpuro-BglII-Hs4E-T_AC

Plasmid

#148445

Purpose

Mammalian Expression of Hs4E-T-shRNA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Hs4E-T-shRNA (EIF4ENIF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB2b

Plasmid

#19470

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB2b (DNAJB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX Nck2-SH2

Plasmid

#46458

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

NM_001004720.2 (NCK2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 274-380

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-hASUN

Plasmid

#47955

Purpose

Expression of tagged-human ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2013 Jul 31.

Insert

Asunder (INTS13 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Sp6

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pBABE human IRS-1 CAAX

Plasmid

#11237

Depositor

Ronald Kahn

Article

Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

Insert

IRS-1 (IRS1 Human)

Use

Retroviral

Tags

CAAX

Expression

Mammalian

Mutation

C-terminal prenylation motif makes IRS-1 localize…

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pGEX p85b(NC)-SH2

Plasmid

#46468

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(NC) (PIK3R2 Bovine)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-717

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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R4pGWB401+Citrine

Plasmid

#128432

Purpose

Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Type

Empty backbone

Use

Gateway destination vector

Tags

Citrine, yellow fluorescence protein

Expression

Plant

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR-Kras

Plasmid

#84637

Purpose

Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras

Use

Tags

6xHis, C-terminal targeting sequence from Kras, C…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Gamma 5

Plasmid

#42200

Depositor

Narasimhan Gautam

Article

Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

Insert

Gamma 5 (Gng5 Rat)

Use

Tags

Venus(1-155)

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Vrap-SH2

Plasmid

#46531

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vrap (SH2D2A Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 93-192

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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vsv-CenpB 1-166-Sgo1 N61I-GFP

Plasmid

#108499

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Tem6-SH2

Plasmid

#46523

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tem6 (TNS3 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1171-1291

Promoter

Tac

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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ETV7_A86D_pet28a

Plasmid

#131657

Purpose

Bacterial expression of ETS gene with A86D mutation for solubility

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 A86D (ETV7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Alanine 86 to Aspartic Acid

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET19b-hMED9

Plasmid

#15365

Depositor

Joan Conaway, Ronald Conaway

Article

Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

hMED9 (MED9 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pTNos:BASTA:PNos (GB0023)

Plasmid

#68211

Purpose

Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

bar gene

Use

Unspecified

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

PNos

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-plk4

Plasmid

#136837

Purpose

Mammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-plk4 (PLK4 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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