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Plasmid#134260PurposeLentivector encoding Flag-tagged GK5 (isoform 2)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5_v1
Plasmid#134257PurposeLentivector encoding Flag-tagged GK5 (isoform 1)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 isoform 1; 516 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-DNMT3L [N117/9.5R]
Plasmid#114514PurposeMammalian Expression Plasmid of anti-DNMT3L (Human). Derived from hybridoma N117/9.5.DepositorInsertanti-DNMT3L (Homo sapiens) recombinant mouse monoclonal antibody (DNMT3L Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795A
Plasmid#114061Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795APromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-TRIP8b (exon 1a/5) [N291C/22R]
Plasmid#114563PurposeMammalian Expression Plasmid of anti-TRIP8b (exon 1a/5) (Mouse). Derived from hybridoma N291C/22.DepositorInsertanti-TRIP8b (exon 1a/5) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-FLAG-TEV
Plasmid#105809PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSico shMAP3K2 mouse
Plasmid#85657Purposeexpression of shRNA targeting mouse MAP3K2DepositorInsertshRNA targeting 3'UTR of Map3k2 (Map3k2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSico shMAP3K2 human
Plasmid#85656Purposeexpression of shRNA targeting human MAP3K2DepositorInsertshRNA targeting 3'UTR of MAP3K2 (MAP3K2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158)-RGS7t
Plasmid#55761PurposeAn amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertRGS7(202-477)-CFP(1-158) (RGS7 Aequorea victoria, Human)
UseTagsCFP(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationThe RGS sequence amino terminal to the GGL domain…PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucA
Plasmid#174047PurposeExpresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinaseDepositorInsertLucA
UseCre/Lox, Lentiviral, and LuciferaseTagsLuciferaseExpressionMammalianMutationALG8 alanine 506 to threoninePromoterHuman Ubiquitin CAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucL
Plasmid#174048PurposeExpresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinaseDepositorInsertLucL
UseCre/Lox, Lentiviral, and LuciferaseTagsLuciferaseExpressionMammalianMutationLAMA4 glycine 1254 to valinePromoterHuman Ubiquitin CAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTripZ EV
Plasmid#209071PurposeDOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector controlDepositorInsertstuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control
UseLentiviralTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv2.1 K+ channel (External) [K39/25R]
Plasmid#128620PurposeMammalian Expression Plasmid of anti-Kv2.1 K+ channel (External) (Human). Derived from hybridoma K39/25.DepositorInsertanti-Kv2.1 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCNB1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.CAP-B10
Plasmid#175004Purposenon-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B10 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
BCL-XL-pcw107-V5
Plasmid#64631Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertBCL2L1 (transcript variant 1) (BCL2L1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kras (G12V)-pcw107
Plasmid#64602Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-hcGAS(157-522)
Plasmid#108676PurposeFor expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522DepositorInsertcGAS amino acid 157-522 (CGAS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acid 157-522PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCag FlpO-2A-Cre EV
Plasmid#129419Purposeepisomal expression of FlpO and Cre recombinasesDepositorInsertFlpO-2A-Cre
UseCre/Lox and Unspecified; Episomal expression vect…TagsExpressionMammalianMutationPromoterCMV/Chick β-actin (CAG)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only