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Plasmid#170725PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsCitrine tag (714bp)ExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PARP2
Plasmid#175810PurposepENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tagsDepositorInsertPARP2 (PARP2 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
VHACg/pDONR221
Plasmid#172094PurposeGenomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221DepositorInsertVHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
UseGateway entry vectorTagsExpressionMutationPromoter530bp upstream of VHA-C ATGAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCCM’ CbbQ-
Plasmid#162711PurposeSecond CCM operon with inactive CbbQ (K46A, E107Q) rubisco activaseDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationInactivation of CbbQ subunit of CbbOQ rubisco act…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHM080Env
Plasmid#139016PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM075Env
Plasmid#139020PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM141Env
Plasmid#139018PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM140Env
Plasmid#139022PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB0405-TEV-His12
Plasmid#137040Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66795.1 (BB_0405 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRevA-TEV-His12
Plasmid#137034Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12DepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pP13-TEV-His12
Plasmid#137035Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM153BEnv
Plasmid#139023PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM026Env
Plasmid#137681PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N entry vector
Plasmid#111751PurposeIntermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cellsDepositorTypeEmpty backboneUseBaculovirus expressionTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB026
Plasmid#119711PurposeTranscriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPromoter gpdA:YFP coding sequence:Terminator trpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL5 sgRNA(MS2)-MS2-hA3a D131A
Plasmid#112131Purposeempty sgRNA cloning vector with MS2-humanA3a D131ADepositorInserthuman Apobec3a D131A mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131A mutant in hA3aPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B2KB46
Plasmid#103086PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB2KB46(Cas9 coding gene from Elusimicrobium minutum (strain Pei191))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-J5TNY4
Plasmid#103116PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertJ5TNY4(Cas9 coding gene from Porphyromonas sp. oral taxon 279 str. F0450)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-K0XCK7
Plasmid#103118PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK0XCK7(Cas9 coding gene from Barnesiella intestinihominis YIT 11860)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q1QGC9
Plasmid#103121PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsert• Q1QGC9(Cas9 coding gene from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only