VHACg/pDONR221
(Plasmid #172094)

• Purpose: Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221
• Depositing Lab: George Haughn
• Publication: Shi et al Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. Epub 2019 Sep 4.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4762
• Total vector size (bp): 7926
• Modifications to backbone: none
• Vector type: Gateway Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon
• Alt name: DET3
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 3225
• GenBank ID: NC_003075.7
• Entrez Gene: DET3 (a.k.a. AT1G12840, ARABIDOPSIS THALIANA VACUOLAR ATP SYNTHASE SUBUNIT C, ATVHA-C, DE-ETIOLATED 3, F13K23.9, F13K23_9, VACUOLAR ATP SYNTHASE SUBUNIT C)
• Promoter: 530bp upstream of VHA-C ATG

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13For
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • VHA-C Gateway fragment

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA