Addgene

We narrowed to 5,213 results for: pCas

Showing: 881 - 900 of 5213 results

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pX551-CMV-SpCas9

Plasmid

#107024

Purpose

AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab

Depositor

Alex Hewitt

Insert

SpCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 neo

Plasmid

#104996

Purpose

This lentiviral construct delivers hSpCas9 and G418 resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG mito-RCaMP1h

Plasmid

#105013

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCI flag PCAF

Plasmid

#8941

Depositor

Yoshihiro Nakatani

Article

Yang et al Nature. 1996 Jul 25. 382(6589):319-24.

Insert

PCAF (KAT2B Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-CD63

Plasmid

#194989

Purpose

overexpress CD63

Depositor

Angelique Bordey

Insert

GFP-CD63

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mEos2

Plasmid

#168515

Purpose

Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-mEos2

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCap-101-Hgy

Plasmid

#227508

Purpose

Capture plasmid with hygromycin resistance and pIJ101 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mNeonGreen-CCR5TC

Plasmid

#135534

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-mNeonGreen-CCR5TC

Plasmid

#135533

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-FBP17

Plasmid

#178366

Purpose

Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

400 Glutamic Acid changed to Aspartic acid (403 A…

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-FBP17

Plasmid

#178365

Purpose

Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCap-101-Apr

Plasmid

#227507

Purpose

Capture plasmid with apramycin resistance and pIJ101 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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SpCas9-ABE8e(V106W)

Plasmid

#198547

Purpose

Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-ABE8e(V106W)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sg1-sg2

Plasmid

#192479

Purpose

All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Use

Tags

T2A mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2-mut

Plasmid

#192783

Purpose

control probe of G-Flamp2, insensitive to cAMP

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2-mut

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-YE1-BE4max

Plasmid

#198546

Purpose

Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-YE1-BE4max

Plasmid

#198535

Purpose

Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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