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Plasmid#222860PurposeThis plasmid codes for the Slug-KH nickase with a sg(GCA)6DepositorInsertSlug-KHD10A+sgGCA
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)
Plasmid#225755PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
EphA1-SAM-Cry2olig
Plasmid#176600PurposeEphrinA1 RTK fusion to Cry2olig - includes SAM domainDepositorInsertEphrinA1-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
EphA1-RTK-Cry2olig
Plasmid#176601PurposeEphrinA1 RTK fusion to Cry2olig - RTK domain onlyDepositorInsertEphA1rtk-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-MARF1 isoform D (HK56)
Plasmid#206470PurposeMARF1 expression in Schneider cells for imagingDepositorInsertMARF1 (CG17018 Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1-GeneTRAP
Plasmid#218184PurposeTo KO and genetrap mouse Hevin simultaneouslyDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1 KO
Plasmid#218182PurposeTo KO Hevin in astrocytesDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0- Gfap-TagIN-mCherry
Plasmid#196490PurposeTo tag endogenous mouse GFAP with mCherryDepositorInsertsmCherry
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-GeneTRAP
Plasmid#196492PurposeTo KO and genetrap mouse GFAP simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-GeneTRAP
Plasmid#196493PurposeTo KO and genetrap mouse VAMP2 simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-TagIN-HA
Plasmid#196494PurposeTo tag endogenous mouse VAMP2 with HADepositorInsertsHA
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-5
Plasmid#221479PurposeGateway cloning of NKX2-5 overexpressionDepositorInsertNKX2-5 (NKX2-5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-NELFE
Plasmid#222559PurposePiggyBac transposon plasmid for doxycycline inducible expression of NELFEDepositorInsertNELFE (NELFE Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TET1
Plasmid#222572PurposePiggyBac transposon plasmid for doxycycline inducible expression of TET1DepositorInsertTET1 (TET1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-PAX6b
Plasmid#222577PurposePiggyBac transposon plasmid for doxycycline inducible expression of PAX6bDepositorInsertPAX6b (PAX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-PAX6a
Plasmid#222576PurposePiggyBac transposon plasmid for doxycycline inducible expression of PAX6aDepositorInsertPAX6a (PAX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-PAX5
Plasmid#222575PurposePiggyBac transposon plasmid for doxycycline inducible expression of PAX5DepositorInsertPAX5 (PAX5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TET3
Plasmid#222574PurposePiggyBac transposon plasmid for doxycycline inducible expression of TET3DepositorInsertTET3 (TET3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-MYBL1
Plasmid#222547PurposePiggyBac transposon plasmid for doxycycline inducible expression of MYBL1DepositorInsertMYBL1 (MYBL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SMAD4
Plasmid#222573PurposePiggyBac transposon plasmid for doxycycline inducible expression of SMAD4DepositorInsertSMAD4 (SMAD4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only