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pFastBac-CHD7
Plasmid#170141PurposeExpresses CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTags6xHis and FLAGExpressionInsectMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
nls-EGFP
Plasmid#67652PurposeMammalian expression vector driving nls-EGFP.DepositorInsertnls-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-cilia-APEX
Plasmid#73186PurposeExpresses cilia-APEX in mammalian cellsDepositorInsertcilia-APEX (Nphp3 Glycine max, Mouse)
UseTagsinternal GFP tagExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-HypaCas9-mRuby2
Plasmid#183872PurposepX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.DepositorTypeEmpty backboneUseCRISPRTagsHypaCas9-P2A-mRuby2-T2A-PuroExpressionMammalianMutationPromoterCbhAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1512
Plasmid#62366PurposeN-terminal 3X FLAG fused to wt BirA - mCherry - sec61ßDepositorInsert3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpTRE2Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
EpsilonCOP EGFP
Plasmid#128151PurposeExpression of epsilon COPDepositorInsertepsilon COP (COPE Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-AAVS1_sgRNA
Plasmid#183890PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.DepositorInsertAAVS1 sgRNA spacer (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(WT)
Plasmid#84639PurposemCherry-tagged, wild-type LKB1DepositorInsertmCherry-LBK1 (STK11 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG
Plasmid#176545PurposeExpresses IgG affinity protein FB with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange glutamic acid 25 to amber stop codonPromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-AAVS1_sgRNA
Plasmid#183891PurposepX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.DepositorInsertAAVS1 sgRNA spacer (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-rtTA
Plasmid#139480PurposeExpresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.DepositorInsertrtTA, Tet-ON reverse transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1a promoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorInsertZinc finger protein 57 (ZNF57 Human)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB80-2xFKBP-L-mCherry-CAAX
Plasmid#193713PurposeRapalog-inducible coupling to plasma membrane (CAAX) via FKBPDepositorInsert2xFKBP-L-mCherry-CAAX
UseTags2xFKBP-mCherryExpressionMammalianMutationmCherry: Met1 DeletedPromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASt-GBP-Trbo
Plasmid#179996PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-EF(N)-10nmSAH-GFP(1-10)
Plasmid#61660Purpose10nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-10nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA0055
Plasmid#131774PurposeGuide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer CASS5a guide (gCASS5a) and 5' sgRNA
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
secCitrine
Plasmid#181964PurposeBasic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertsignal peptide sequence from basic chitinase
UseTagsCitrine YFPExpressionPlantMutationPromoter1.5 kb MUM4 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only
