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Plasmid#227893PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCLTA homology arms with mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN R255E-HA
Plasmid#186894PurposeDoxycycline-dependent expression of human cGAS gene (with R255E mutation) in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with R255E mutation and C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsHAExpressionMutationN-terminal truncation, R255EPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-FLAG-human cGASdeltaN-HA
Plasmid#186899PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsFLAG and HAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-mouse cGASdeltaN-GFP
Plasmid#186878PurposeDoxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transductionDepositorInsertMouse cGAS truncation mutant (148-508 a.a.) with C-terminal GFP fusion (Cgas Mouse, Synthetic)
UseRetroviralTagsGFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-GFP-human cGASdeltaN
Plasmid#186880PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal GFP fusion (Adamts1 Human, Synthetic)
UseRetroviralTagsGFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN-OMM
Plasmid#186881PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain (503-541 a.a.) fusion (Adamts1 Human, Synthetic)
UseRetroviralTagsExpressionMutationN-terminal truncation, MAVS transmembrane domain …PromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN-Pex
Plasmid#186882PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal PEX13 transmembrane domain (136-233 a.a.) fusion (Adamts1 Human, Synthetic)
UseRetroviralTagsExpressionMutationN-terminal truncation, PEX13 transmembrane domain…PromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-rhesus macaque cGASdeltaN-HA
Plasmid#186866PurposeDoxycycline-dependent expression of rhesus macaque cGAS gene in mammalian cells by retroviral transductionDepositorInsertRhesus macaque cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (CGAS Macaca mulatta, Synthetic)
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN-GFP
Plasmid#186845PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal GFP fusion (Adamts1 Human, Synthetic)
UseRetroviralTagsGFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-orangutan cGASdeltaN-GFP
Plasmid#186846PurposeDoxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)
UseRetroviralTagsGFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-chimpanzee cGASdeltaN-GFP
Plasmid#186847PurposeDoxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transductionDepositorInsertChimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal GFP tag (CGAS Pan troglodytes, Synthetic)
UseRetroviralTagsGFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-FLAG-human cGASdeltaN-HA
Plasmid#186848PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsFLAG and HAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP1delta103CSA11-P2A-GFP
Plasmid#220814PurposeExpress separately the Rotavirus SA11 delta103C NSP1 protein and the sfGFP (Pedelacq et al., 2006)DepositorInsertRotavirus SA11 NSP1delta103C-P2A-GFP
UseOtherTagsExpressionMutationLacks C-terminal 103 aaPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-GABA(A)R, Delta [N151/3R-2b]
Plasmid#206657PurposeMammalian Expression Plasmid of anti-GABA(A)R, Delta (Rat). Derived from hybridoma N151/3-2b.DepositorInsertanti-GABA(A)R, Delta (Rattus norvegicus) recombinant Mouse monoclonal antibody (Gabrd Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Amber-free HIV-1 deltaRT
Plasmid#213007PurposeAn replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.DepositorInsertsHIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
Env BG505
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-SLD1
Plasmid#210264PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-SLD1PromoterCMV/TetO2Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-SLD2
Plasmid#210263PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-SLD2PromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Delta scFv [N151/3]
Plasmid#206724PurposeMammalian Expression of GABA(A)R, Delta scFV. Derived from hybridoma N151/3 scFv.DepositorInsertGABA(A)R, Delta (Rattus norvegicus) recombinant scFV (Gabrd Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-MVP delta607-623aa
Plasmid#204542PurposeExpresses GST-tagged MVP delta607-623aa in E.coliDepositorInsertMVP (MVP Human)
UseTagsExpressionBacterialMutationdelta607-623aaPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Atg32-deltaPsR pXP721
Plasmid#200735PurposePlasmid for the expression of GFP-Atg32 lacking the PsR domain in S. cerevisiae.DepositorInsertAtg32
UseTagsGFPExpressionYeastMutationAmino acids 200-341 are deleted.PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only