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Plasmid#158516PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA
UseTags6xHAExpressionPlantMutationD481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA46
Plasmid#158524PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationD481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA47
Plasmid#158525PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA48
Plasmid#158526PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationL17E, D481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
as-mOPN-PB0
Plasmid#106450PurposeBacterial expression of antisense to murine osteopontinDepositorInsertantisense-osteopontin (Spp1 Mouse)
UseRetroviralTagsExpressionMutationantisensePromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1F
Plasmid#135660PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1R
Plasmid#135661PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
P302-FBP_1
Plasmid#139692PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P313-FBP_8
Plasmid#139699PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3HDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-cimA3.7
Plasmid#134595PurposeLow Phosphate inducible mutant citramalate synthaseDepositorInsertcimA3.7
UseTagsExpressionBacterialMutationI47V, E114V, H126Q, T204A, L238SPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-SBS-mCherry
Plasmid#134598PurposeLow Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)DepositorInsertSBS-mCherry
UseTagsmCherryExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-T7-CBD-hGLY
Plasmid#134940PurposeT7 based expression of human glycine acyltransferase with an N-terminal chitin binding domainDepositorInserthuman glycine acyltransferase 2 (GLYATL2 )
UseTagschitin binding domainExpressionBacterialMutationPromotert7Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY921
Plasmid#135092PurposeTranscriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegansDepositorInsertPacr-2
UseTagsGFP and Synthetic IntronExpressionWormMutationPromoterPacr-2Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY847
Plasmid#135093PurposeTranscriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegansDepositorInsertPacr-2
UseTagsmcherry and unc-10 3'UTRExpressionWormMutationPromoterPacr-2Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-C3H
Plasmid#133386PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-B3HTK
Plasmid#133389PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTNG5kwh
Plasmid#128279PurposeMammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion proteinDepositorInserthTetR::NLS::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsEGFPExpressionMammalianMutationPromoterCMV-D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet139
Plasmid#121913PurposeCharge-Introduced NpuDnaB mini-inteinDepositorInsertCI-NpuDnaBΔ290 intein
UseTagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet140
Plasmid#121914PurposeOrthogonal NpuDnaB mini-intein inteinDepositorInsertOth-NpuDnaBΔ290 intein
UseTagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only