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Plasmid#91068PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
RF15
Bacterial Strain#102799PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.DepositorBacterial ResistanceNoneAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B
Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1
Plasmid#205447Purposehuman AAVS1-targeting plasmid for expression of mouse CR1DepositorInsertmCR1
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha Wildtype
Plasmid#163365PurposeExpress HA- and Clover-tagged HIF-1alpha wildtype plasmidDepositorInsertHypoxia Inducible Factor 1 alpha (HIF1A Human)
UseTagsHA- and Clover-taggedExpressionMammalianMutationWildtypePromoterCMV PromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W
Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-Sox2-Pur
Plasmid#16577DepositorInsertHomo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ATG16L1_WT
Plasmid#82255PurposeGateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.DepositorInsertATG16L1 (ATG16L1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC1A2_STOP
Plasmid#161067PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC1A2 (SLC1A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Anillin
Plasmid#68027PurposeFull length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1DepositorInsertAnillin (ANLN Human)
UseTagseGFPExpressionMammalianMutationsilent mutations at 798 5'-TGCC TCTTTGAATAAA…PromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC1A4_STOP
Plasmid#161128PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC1A4 (SLC1A4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HisMBP
Plasmid#11085DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and MBPExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
UseTagsExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…PromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCWX-UBI-hTert-PGK-BSD
Plasmid#114316PurposeConstitutive expression of the hTert proteinDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCAS GFP (CT#22)
Plasmid#13878DepositorInsertGFP
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-hEAAT2
Plasmid#32814DepositorInserthEAAT2 (SLC1A2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human Igf2bp2-IRES-GFP
Plasmid#91890PurposeRetroviral expression of human Igfbp2DepositorInsertIgf2bp2 (IGF2BP2 Human)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ2072.2_CRISPRlator
Plasmid#140674PurposeCRISPRlatorDepositorInsertCRISPRlator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3132
Plasmid#144608PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertESR2 (ESR2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only