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Plasmid#126899PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ WRKY28_2
Plasmid#126900PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ PLDbeta1
Plasmid#126902PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ OBfold
Plasmid#126901PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL4723_ OBfold
Plasmid#126889PurposeGenome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ HDT701
Plasmid#126898PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ Dja6_1
Plasmid#126894PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ ERF922
Plasmid#126893PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
FFTTH-Amotl2
Plasmid#112832Purposetransient/stable overexpressionDepositorInsertAmotl2 (Amotl2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC37
Plasmid#104811PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from Gmubi promoterDepositorInsertMedtr3g107390
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK523
Plasmid#72442PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)DepositorInserthypothetical protein
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK126
Plasmid#73337PurposeEncodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 328-685PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK621
Plasmid#72388PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK125
Plasmid#73336PurposeEncodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 1-327PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK553
Plasmid#72452PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK636
Plasmid#72466PurposeProduces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK637
Plasmid#72437PurposeAcetobacter aceti 1023 mutS, partial geneDepositorInsertDNA mismatch repair protein MutS (partial gene)
UseTagsExpressionBacterialMutationdeletes nt 1-767 and nt 2086-2655 of mutS genePromotern/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK620
Plasmid#72537PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK622
Plasmid#72538PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdTurboRFP-Zyxin-6
Plasmid#58067PurposeLocalization: Focal Adhesions, Excitation: 553, Emission: 576DepositorInsertZyxin (ZYX Human)
UseTagstdTurboRFPExpressionMammalianMutationS11P, G42R and E62DPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits