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Plasmid#223177PurposePlant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left borderDepositorTypeEmpty backboneUseTi plasmid for agrobacteriumTagsExpressionPlantMutationPromoterAvailable sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pcDNA5-FRT/T0-EGFP-GNB1L
Plasmid#222621PurposeMammalian expression vector of flag-tagged GNB1LDepositorInsertGNB1L (GNB1L Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-GFAPp-PiGM-Iq(eGFP)
Plasmid#221606PurposeA recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. GFAP promoter for astrocyte-selective expression.DepositorInsertPiGM-Iq (eGFP)
UseAAVTagsExpressionMutationRGS2 1-53 truncationPromoterGFAP promoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2179-FRB-GPA-6-GFPS
Plasmid#222893PurposeBiosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.DepositorInsertFRB-GPA-6GS-GFPS
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MLG-EGFP-LST2
Plasmid#220785PurposeExpresses N-terminal EGFP-LST2 in mammalian cellsDepositorArticleInsertLST2 (ZFYVE28 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cTNT-GFP-Palmd
Plasmid#216832Purposecardiomyocytes-specific plasmid, expressing Gfp and the coding sequence of mouse PalmdDepositorInsertPalmd (mus musculus) (Palmd Mouse)
UseAAVTagsGFPExpressionMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cTNT-NEXN-GFP
Plasmid#216835Purposecardiomyocytes-specific plasmid, expressing the coding sequence of mouse Nexn and GFP tagDepositorInsertNexn (mus musculus) (Nexn Mouse)
UseAAVTagsGFPExpressionMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-PiGM-Iq(eGFP)
Plasmid#221602PurposeA recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. hSynapsin promoter for panneuronal expression.DepositorInsertPiGM-Iq (eGFP)
UseAAVTagsExpressionMutationRGS2 1-53 truncationPromoterHuman SynapsinAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mNINJ1-K45Q-GFP
Plasmid#208777PurposeMSCV vector expressing K45Q mouse NINJ1DepositorInsertNINJ1 (Ninj1 Mouse)
UseRetroviralTagsGFPExpressionMutationChange Lys 45 to Gln (K45Q)PromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mNINJ1-D53A-GFP
Plasmid#208778PurposeMSCV vector expressing D53A mouse NINJ1DepositorInsertNINJ1 (Ninj1 Mouse)
UseRetroviralTagsGFPExpressionMutationChanged aspartic acid 53 to alanine (D53A)PromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-MCS-Puro-EGFP
Plasmid#219795PurposeMammalian expression plasmids using piggyBac Transposon SystemDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-EGFP
Plasmid#220594PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.DepositorInsertEGFP
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP
Plasmid#219828PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-2xMARS-nGFP-puro
Plasmid#205238PurposeLentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobodyDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseLentiviralTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shAqp4-4
Plasmid#223225Purposemir30 based shRNA strategy, shRNA targeting mouse Aqp4DepositorInsertshAqp4 (Aqp4 Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shAqp4-3
Plasmid#223224Purposemir30 based shRNA strategy, shRNA targeting mouse Aqp4DepositorInsertshAqp4 (Aqp4 Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shAqp4-1
Plasmid#223223Purposemir30 based shRNA strategy, shRNA targeting mouse Aqp4DepositorInsertshAqp4 (Aqp4 Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shLacZ
Plasmid#223222Purposemir30 based shRNA strategy, control shRNADepositorInsertshLacZ
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zhou-pLOV-GFP-Luc
Plasmid#161030PurposeLentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection.DepositorInsertGFP and luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only