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Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast
Plasmid#216733PurposeExpresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.DepositorArticleInsertCas9-D10A nickase and sgCTG
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10APromoterU6 and CAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2
Plasmid#108422PurposeAAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expressionDepositorHas ServiceAAV5InsertArchon1-KGC-EGFP-ER2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2K-LRL-mouse c-MYC-IRES-Luc2
Plasmid#109228PurposeFor Tol2 transposon-mediated stable expression of LRL, c-Myc and IRES-luciferaseDepositorInsertc-Myc (Myc Mouse)
UseTagsIRES-Luc2ExpressionMammalianMutationPromoterCAGGSAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-MSCV-hCD38-EF1α-GFP+Puro
Plasmid#134936Purposeexpression human CD38 in mammalian cellsDepositorInsertCD38 (CD38 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv2.1 K+ Channel [D3/71R]
Plasmid#128612PurposeMammalian Expression Plasmid of anti-Kv2.1 K+ Channel (Rat). Derived from hybridoma D3/71.DepositorInsertanti-Kv2.1 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP-Vimentin-17-C
Plasmid#108414PurposeVimentin fused to monomeric near-infrared fluorescent protein miRFPDepositorInsertVimentin (VIM Human)
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-PSD-95 [K28/43R]
Plasmid#128618PurposeMammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/43.DepositorInsertAnti-PSD-95 (Human) recombinant mouse monoclonal antibody (DLG4 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL-MND-hCard11 WT-T2A-GFP
Plasmid#163787PurposeExpression of Wild-type CARD11DepositorInsertCARD11 (CARD11 Human)
UseLentiviralTagsExpressionMutationPromoterMNDAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-PdCO-EGFP
Plasmid#198512PurposeExpresses optimized PdCO in frame with EGFP under control of CMV promotor.DepositorInsertPdCO
UseTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV(gRNA)-CMV-eGFP-U6(sgCTG)
Plasmid#216732PurposeContains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytesDepositorArticleInsertsgCTG lentiviral guide RNA with eGFP tag
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR4-DuET
Plasmid#213221PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR4 (CXCR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ankyrin-R [N388A/10R]
Plasmid#114479PurposeMammalian Expression Plasmid of anti-Ankyrin-R (Human). Derived from hybridoma N388A/10.DepositorInsertanti-Ankyrin-R (Homo sapiens) recombinant mouse monoclonal antibody (ANK1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-GFAP [N206A/8R]
Plasmid#177512PurposeMammalian Expression Plasmid of anti-GFAP (Human). Derived from hybridoma N206A/8.DepositorInsertanti-GFAP (Homo sapiens) recombinant mouse monoclonal antibody (GFAP Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-SUR2B [N323B/20R]
Plasmid#114480PurposeMammalian Expression Plasmid of anti-SUR2B (Rat). Derived from hybridoma N323B/20.DepositorInsertanti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody (Abcc9 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188901PurposedCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertSID-dCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTHC
Plasmid#112620Purposeexpression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2DepositorInsertsUseTagsmTeal (mTFP1)ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-miRFP
Plasmid#108416PurposeMonomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmidDepositorInsertmiRFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-Arl13b [N295B/66R]
Plasmid#140076PurposeMammalian Expression Plasmid of anti-Arl13b (Mouse). Derived from hybridoma N295B/66.DepositorInsertanti-Arl13b (Mouse) recombinant mouse monoclonal antibody (Arl13b Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only