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150,461 results

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Orai1-GCaMP6f

Plasmid

#73564

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

Orai1-GCaMP6f (ORAI1 Human, Synthetic)

Use

Tags

GCaMP6f

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

Plasmid

#159897

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | gfp | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-hHSF1 WT

Plasmid

#71724

Purpose

plasmid expressing myc-his tag hHSF1 WT

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

HSF1 (HSF1 Human)

Use

Tags

MYC 6xHIS

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Mfn1-Myc

Plasmid

#23212

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Use

Tags

6xHis, 9xMyc, and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pAWP78

Plasmid

#61263

Purpose

Small IncP-based broad host range replicating vector.

Depositor

Mary Lidstrom

Article

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H151A; H1121A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HPV16 E6 TCR

Plasmid

#122727

Purpose

Express HPV16 E6 TCR

Depositor

Christian Hinrichs

Article

Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

Insert

HPV16 E6 TCR

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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lenti.Cas9.BFP.Blast

Plasmid

#196714

Purpose

WT SpCas9 with BSD-TagBFP for selection and sorting

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)

Use

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-780

Plasmid

#21307

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagRFP

Plasmid

#67167

Purpose

CRISPaint universal donor for TagRFP fusion tagging

Depositor

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagRFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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-204 human IFN-gamma luc

Plasmid

#17596

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-204 to +38 of promoter

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pCIneo-hCHD7

Plasmid

#89458

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-VSVG

Plasmid

#64084

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

Depositor

Ian Wickersham

Insert

VSV glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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LV-hNG2-GFP

Plasmid

#183910

Purpose

Expression of GFP under the human NG2 promoter

Depositor

Chun-Li Zhang

Article

Tai et al Cell Stem Cell. 2021 May 6;28(5):923-937

Insert

GFP (GFAP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pHDM-SARS-CoV-2 Δ18

Plasmid

#164434

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike truncated to remove 18 amino acids (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mRuby2-FRET-10

Plasmid

#58179

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

Type

Empty backbone

Use

Tags

mNeonGreen and mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA ZIP-GFP11

Plasmid

#182242

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP11

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

ZIP-GFP11

Use

Tags

split-GFP11 tripartite

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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