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Plasmid#70650PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 2
Plasmid#70653PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RTRG
Plasmid#69845PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert1 RTRG repeat
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)2
Plasmid#69846PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert2 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)3
Plasmid#69847PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert3 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)4
Plasmid#69848PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert4 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED55 pRS306 pRPS2-SteND-NLS-mCherry
Plasmid#68277PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutationDepositorInsertSte7ND NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
Plasmid#68275PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLSDepositorInsertSte7DS NLS(4A) mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUNC2001
Plasmid#67280PurposeExpresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcwDepositorInsertVibrio cholera photolyase
UseTagsmaltose binding proteinExpressionBacterialMutationnone knownPromoterAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 R123A
Plasmid#66721PurposeConstitutive expression of FPR1 R123A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationR123APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 D71A
Plasmid#66720PurposeConstitutive expression of FPR1 D71A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationD71APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1 R4-R3
Plasmid#62105PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIhh4.5k-Luc
Plasmid#53603PurposeReporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-ERr
Plasmid#61171PurposeFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under MtBCP1 promoterDepositorInsertAtWAK2-mCherry-HDEL
UseTagsExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1 L1-L4
Plasmid#62104PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHYBAD94
Plasmid#42305DepositorInsertNpuDnaEC6
UseTagsGB1-H6ExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1 L1-R5
Plasmid#62101PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-ADAM21i1
Plasmid#59604PurposeExpression of shRNA against human ADAM21DepositorInsertADAM21 (ADAM21 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-CENPBi2
Plasmid#59619PurposeExpression of shRNA against human CENPBDepositorInsertCENPB (CENPB Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-SLC17A5i1
Plasmid#59598PurposeExpression of shRNA against human SLC17A5DepositorInsertSLC17A5 (SLC17A5 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only