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pLenti-CMV-MCS-GFP-SV-puro
Plasmid#73582PurposeThird generation lentiviral vector expressing GFP and puromycinDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsGFP tagExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Capping Protein
Plasmid#89950PurposeF-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.DepositorInsertsUseTags6*HisExpressionBacterialMutationV222IPromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC1177-pHR-SFFV-GFP(1-10)-NLS
Plasmid#164039PurposeExpression of GFP1-10 in mammalian cellsDepositorInsertGFP(1-10)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
DPYSL2-pLJC2
Plasmid#189866PurposeExpresses human DPYSL2 (CRMP2) in mammalian cellsDepositorInsertDPYSL2 (DPYSL2 Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-2xFlag
Plasmid#102931PurposeExpress a lysosomal TagDepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6-5'+3' MS2-CircRNA
Plasmid#213752PurposeFor circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cellsDepositorInsert5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV8)
Viral Prep#104492-AAV8PurposeReady-to-use AAV8 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK603
Plasmid#8831DepositorInsertTEV protease, S219D mutant
UseTagsExpressionBacterialMutationS219D mutantPromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG:GPI-GFP
Plasmid#32601DepositorInsertGPI-GFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIneo RYR-1
Plasmid#75113PurposeClone encoding rabbit ryanodine receptor in mammalian expression vectorDepositorInsertRyanodine receptor 1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC70196
Plasmid#209457PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/intron/ARE
Plasmid#62858Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationinsertion of chimeric intron at nueotide poclsiti…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Taq DNA Pol
Plasmid#178782PurposeInducible expression of Taq DNA polymerase in E. coliDepositorInsertTaq DNA Polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA-mut (AAV9)
Viral Prep#140558-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA-mut (#140558). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA-mut plasmid DNA. Synapsin-driven expression of control red fluorescent dopamine sensor GRAB_rDA-mut (does not respond to DA). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
gGBEv6.3-SpG
Plasmid#202630Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpG(D10A)-MPGv6.3
UseTagsExpressionMammalianMutationSpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…PromoterhU6, EF1a, CBhAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only