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Plasmid#184893PurposeStable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cellsDepositorInsertANXA7 (ANXA7 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0090
Plasmid#142456PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xHA-TurboID_pAS31
Plasmid#118220Purposeexpresses 3xHA-tagged TurboID in C. elegans intestineDepositorInsertTurboID (BirA mutant)
UseTags3x HA tagExpressionWormMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterges-1pAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-TC
Plasmid#211733PurposeExpresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsTetracysteineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mCherry LIC cloning vector (6B)
Plasmid#30125PurposeEmpty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PDGFRB
Plasmid#116770PurposeLentiviral expression of PDGFRBDepositorInsertPDGFRB (PDGFRB Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-CLDN18
Plasmid#69466PurposeExpresses CLDN18DepositorInsertCLDN18 (CLDN18 Human)
UseRetroviralTagsHA TAGExpressionMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.28.3z
Plasmid#200678PurposeModular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_miRFP670
Plasmid#113726PurposeExpresses the fluorescent protein miRFP670 in a lentiviral backboneDepositorInsertmiRFP670
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Optimized UBE1L
Plasmid#165099Purposeecoli optimized expression of GST-fusion of human Ube1LDepositorInsertUbeE1L (UBA7 Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorInsertsI-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-yeastSMASh-YFP
Plasmid#68854PurposeExpresses SMASh tag fused to YFP at N terminus in yeastDepositorInsertyeastSMASh-YFP
UseTagsSMASh tagExpressionYeastMutationPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804DepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRDA_526
Plasmid#184859PurposeLentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-HNF4α
Plasmid#139305PurposeKnocking down of human HNF4α through shRNA and amiRNA targeting HNF4αDepositorInsertshRNA and amiRNA against HNF4α
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GFP10-ZIP
Plasmid#182241PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP10DepositorInsertGFP10-ZIP
UseTagssplit-GFP10 tripartiteExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL
Plasmid#34593DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pLAT1_Cas9
Plasmid#104907PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
RN3P-KDM5B-FLAG
Plasmid#86398PurposeFLAG-tagged mouse KDM5B cDNA clone for mammalian cell expressionDepositorInsertKDM5B (Kdm5b Mouse)
UseTags3x-FLAGExpressionMammalianMutationPromoterLacAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only