Addgene

We narrowed to 1,095 results for: piggyBac

Showing: 861 - 880 of 1095 results

Results per page:

pB-Ric8b-Integration

Plasmid

#129457

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

Ric8b (Ric8b Mouse)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE (Tet-On)

Availability

Academic Institutions and Nonprofits only

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pB-RTP2-Integration

Plasmid

#129459

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

RTP2 (RTP2 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE (Tet-On)

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-CTNNB1_S33Y

Plasmid

#133376

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

CTNNB1 (CTNNB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S33Y

Promoter

CMV enhancer and hEf1a (CpG free)

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-dnTCF4-ires-GFP

Plasmid

#133378

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

dnTCF4 (TCF4 Human)

Use

Tags

2x Flag

Expression

Mammalian

Mutation

deltaN31

Promoter

CMV enhancer and hEf1a (CpG free)

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW-IGF2

Plasmid

#133383

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IGF2 (IGF2 Human)

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Availability

Academic Institutions and Nonprofits only

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pB-TAG-HES7

Plasmid

#130935

Purpose

PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Use

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AcKRS

Plasmid

#140021

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AcKRS-3 (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L301M, L305I, Y306F, L309A, C348F

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-PcKRS

Plasmid

#140022

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

PcKRS (pylS Methanosarcina bakeri)

Use

Tags

FLAG

Expression

Mammalian

Mutation

M241F, A267S, Y271C, L274M

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ECC-Biosensor

Plasmid

#140832

Purpose

Encoding the CAG promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

ECC-Biosensor

Use

Piggybac (pb) transposon

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-ECC-Biosensor

Plasmid

#140833

Purpose

Encoding the TRE promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

TRE-ECC Biosensor

Use

Piggybac (pb) transposon

Tags

Expression

Mammalian

Mutation

Promoter

inverse

Availability

Academic Institutions and Nonprofits only

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PB-TetR-tdTomato

Plasmid

#212958

Purpose

piggyBac donor vector containing TetR-tdTomato for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

TetR-tdTomato

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-LacI-eGFP

Plasmid

#213021

Purpose

piggyBac donor vector containing LacI-eGFP for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

LacI**-eGFP

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-CAG-dCasRx-RBM25-IRES-BleoR

Plasmid

#221002

Purpose

Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

dCasRx-RBM25-IRES-BleoR

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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iOn-CAG∞RFP

Plasmid

#154014

Purpose

Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞GFP

Plasmid

#154015

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞RFP

Plasmid

#154016

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Use

Tags

Expression

Mammalian

Mutation

H210P

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞IRFP

Plasmid

#154017

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

IRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3x127D01-DsRed

Plasmid

#171578

Purpose

For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-N-3x127D01-DsRed

Plasmid

#171579

Purpose

For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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