Addgene

We narrowed to 14,943 results for: grna

Showing: 861 - 880 of 14943 results

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pLsCas13a-gRNA-3

Plasmid

#164867

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-3

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pT7gRNA-ccdb

Plasmid

#140864

Purpose

Backbone for making sgRNAs.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_stgRNA-Puro

Plasmid

#164124

Purpose

Backbone for lentiviral stgRNA library construction (library 1 and 2)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pheT

Plasmid

#154920

Purpose

aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pheT

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-mqo

Plasmid

#154921

Purpose

aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting mqo

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisF

Plasmid

#154922

Purpose

aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting hisF

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ampG

Plasmid

#154923

Purpose

aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting ampG

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAflaB

Plasmid

#149561

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1sgRNA2

Plasmid

#160946

Purpose

Guide RNA 2 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PflaBS-sgRNAflaB

Plasmid

#149643

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, PflaBS

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNArodA

Plasmid

#149569

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0826S-sgRNA500

Plasmid

#149618

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

P0826S

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826L-sgRNAflaB

Plasmid

#149648

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, P0826L

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTL-sgRNA500

Plasmid

#149617

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PresTL

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_H19-DMR-2

Plasmid

#159930

Purpose

Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse H19-DMR (H19-icr Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-1

Plasmid

#159931

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_H19-DMR-1

Plasmid

#159929

Purpose

Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse H19-DMR (H19-icr Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNA500

Plasmid

#149559

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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