-
Plasmid#176724PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Eremothecium cymbalariae (receiver part).DepositorArticleInsertste2Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK030
Plasmid#176734PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Candida albicans (barrier part).DepositorArticleInsertbar1Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK031
Plasmid#176735PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Eremothecium cymbalariae (barrier part).DepositorArticleInsertbar1Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK008
Plasmid#176712PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Candida albicans (sender part).DepositorArticleInsertmfalpha1Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK034
Plasmid#176738PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).DepositorArticleInsertbar1Sc
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB255
Plasmid#170038PurposeEncodes LacI-controlled expression of surface-displayed melittin and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Melittin
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSG74-β2AR-sfGFP
Plasmid#102460PurposeExpresses a fusion protein of wild type β2 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ2AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tTA
Plasmid#149363PurposeAAV-mediated and Cre-dependent expression of tTA under the CAG promoter.DepositorInserttTA2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW012
Plasmid#131815PurposeVector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55DepositorArticleInsertM. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV_CAG_Gag
Plasmid#212634PurposeLentivirus vector to express MLV GagDepositorArticleInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UseLentiviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2699-CMV-Nme2Cas9-E932R
Plasmid#228720PurposeExpresses Nme2 Cas9 nuclease with E932R mutation in mammalian cellsDepositorInsertNme2Cas9-E932R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2698-CMV-Nme2Cas9-D56R
Plasmid#228719PurposeExpresses Nme2 Cas9 nuclease with D56R mutation in mammalian cellsDepositorInsertNme2Cas9-D56R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_coGag
Plasmid#212656PurposepiggyBac vector to express codon optimized MLV GagDepositorArticleInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGag-mCherry
Plasmid#212631PurposeExpression vector for an MLV Gag-mCherry fusion proteinDepositorArticleInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_EF1a_Gag
Plasmid#212638PurposeLentivirus vector to express MLV GagDepositorArticleInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-Puro
Plasmid#227262PurposeEmpty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-Blast
Plasmid#227263PurposeEmpty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2
Plasmid#222857PurposeThis plasmid codes for the Slug-KH nickase with a sg(CTG)6DepositorInsertSlug-KHD10A+sgCTG
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2
Plasmid#222859PurposeThis plasmid codes for the Slug-KH nickase with a sg(AGC)6DepositorInsertSlug-KHD10A+sgAGC
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2
Plasmid#222858PurposeThis plasmid codes for the Slug-KH nickase with a sg(CAG)6DepositorInsertSlug-KHD10A+sgCAG
UseCRISPRTags3X FLAG, SV40 NLS, ITR2 and ITR2ExpressionMammalianMutationKH variant and D10A mutationPromoterH1 and eCMVAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only