-
Plasmid#170277PurposeExpression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)DepositorInsertBst-LF D720A
UseTagsHexahistidineExpressionBacterialMutationD720APromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo
Plasmid#170319PurposeExpresses HA and EGFP tagged RPL22, neomycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2 SH2-SH3
Plasmid#46442DepositorInsertGrb2 (GRB2 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 1-217PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX
Plasmid#163554PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-Anc689-UdgX-NG-nCas9-RBMX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorUseTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-oScarlet
Plasmid#137138PurposeIntersectional viral expression of oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Hck-SH2
Plasmid#46445DepositorInsertHck (HCK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 134-254PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330-Elf5 gRNA
Plasmid#128836PurposegRNA for targeting mouse Elf5 loci using CRISPR-cas techniqueDepositorInsertmElf5 gRNA (Elf5 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL
Plasmid#111391PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mRuby2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta2-3xFiBsd
Plasmid#140964Purposefull-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis-associated gene family, member 2 (Mta2 Mouse)
UseTags3xFLAG, Avi, and TEVExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP
Plasmid#102594PurposeLentivector to express STING V155M delta 342 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationV155M delta 342PromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP
Plasmid#137146PurposeIntersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT FlpDepositorInsertCon/Foff 2.0-bREACHes-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-CD79A-RFP
Plasmid#187003PurposeExpression of human CD79A as RFP fusion proteinDepositorInsertCD79A (CD79A Human)
UseLentiviralTagstagRFPExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9-RBMX
Plasmid#163565PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9-RBMX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BEACON1
Plasmid#171697PurposeExpresses BEACON1 in mammalian cellsDepositorInsertBEACON1
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_W104A/Y132DPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC4 Frk-SH2
Plasmid#46428DepositorInsertFrk (FRK Human)
UseTagsAviTag, GST, and Thrombin siteExpressionBacterialMutationcontains amino acids 108-213PromoterTrcAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_FNDC3B
Plasmid#45269DepositorInsertFNDC3B (FNDC3B Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-RPS4X-3xHA plasmid
Plasmid#192246Purposeoverexpression vector of RPS4X-3XHADepositorInsertRPS4X CDS without stop coden (RPS4X Human)
UseLentiviralTags3xHAExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP6
Plasmid#128940PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, ZDepositorInsertsUseTagsHisExpressionBacterialMutationPreScission protease site (GE Healthcare; LEVLFQG…PromoterT7Available sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC591 - pCMV Mem-iRFP
Plasmid#59134PurposeA plasmid that express membrane-targeted iRFP under the CMV promoter.DepositorInsertMembrane-localized Infrared Fluorescent Protein
UseTagsPalmitylation signalExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only