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Plasmid#119278Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-nsp5
Plasmid#168868PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-nsp5 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE
Plasmid#139087PurposeExpresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.DepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsExpressionMammalianMutationL1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…PromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1582
Plasmid#154312PurposemNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmNeonGreen^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p221z-erTagRFP
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP712
Plasmid#65768PurposeBacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNADepositorInsertmammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
UseCRISPRTags3x FLAG and NLSExpressionBacterialMutationD10A and H840A mutations in Cas9PromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mCerulean
Plasmid#185009PurposeFor knocking in FKBP F36V P2A mCeruleanDepositorInsertFKBP F36V P2A mCerulean
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP1358
Plasmid#119277Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-ORF8
Plasmid#168884PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-ORF8 (ORF8 )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1354
Plasmid#119275Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-HKU1-Spike
Plasmid#168911PurposeGateway-compatible Entry vectorDepositorInsertHCoV-HKU1-Spike (S )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-GAP43-ECFP-stop-L2
Plasmid#186356PurposeEntry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequencesDepositorInsertGrowth-associated protein-43 (Gap43 Mouse, Synthetic)
UseExpression of a fluorescent membrane markerTagsECFPExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF160
Plasmid#135925PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mAzurite
UseTransposonTagsmAzuriteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU3b/LacZ
Plasmid#66199Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAtU3bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pROS10
Plasmid#107924PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #1
Plasmid#174511PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.DepositorInsert[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pep…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-VPS35
Plasmid#21689DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseGateway entry cloneTagsExpressionMutationlast nucleotide of stop codon removed for c-termi…PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHM106Env
Plasmid#139024PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEL17
Plasmid#107923PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only