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Plasmid#78304PurposeCreating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-nlsLexA::GADfluw-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
mActZ-FY
Plasmid#171463PurposeExpresses mActZ-FY, a FiCE of iCMM, in mammalian cellsDepositorInsertActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-EYFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mActZ-FmCh
Plasmid#171465PurposeExpresses mActZ-FmCh, a FiCE of iCMM, in mammalian cellsDepositorInsertActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ179
Plasmid#85996PurposeOne-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2DepositorInsertsEGFP-NT2_cr2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified mouse U6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OS
Plasmid#136576PurposeDox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2DepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3
Plasmid#49208Purposeexpresses Flag tagged mouse Tim3DepositorInsertTim3 (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationno Tim3 signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xML1N(7Q)
Plasmid#67666PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1Nmut*2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 WT
Plasmid#182046Purposeexpression of Sox2DepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N450
Plasmid#92321PurposeN-term 1-450 of HCF-1 proteinDepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-450. Note that a SRRASGTYGRV amino-terminal …PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APPSwed/Ind
Plasmid#114194PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
UseTagsExpressionMammalianMutationPopular mouse model, overexpress 2 mutations: Swe…PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXF2
Plasmid#153123PurposeExpression of Flag tagged human FOXF2 in mammalian cellsDepositorInserthuman FOXF2 (FOXF2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-sfGFP-SKL-Target2 (Mpphot)
Plasmid#186722PurposeTransient expression vector for SKLPT imaging for target 2 (Mpphot) in plant cells (Negative control vector).DepositorInsertsfGFP-SKL-Mphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2
Plasmid#174817PurposeMammalian expression of SaCas9 prime editor 2DepositorInsertSaPE2
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST JNKKTRmRuby2
Plasmid#59154PurposeLentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human)
UseLentiviralTagsmRuby2ExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMJ114
Plasmid#85995PurposeOne-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant regionDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified bovine U6-2Available sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf ctnna1L344P
Plasmid#185407PurposeZebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMVDepositorInsertctnna1 (ctnna1 Zebrafish)
UseIn vitro synthesis of mrnaTagsExpressionMammalianMutationchanged Leucine 344 to ProlinePromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cav3P104L-YFP
Plasmid#68404PurposeExpression of fluorescently tagged Caveolin3 point mutation in mammalian cellsDepositorInsertCav3 (Cav3 Mouse)
UseTagsEYFPExpressionMammalianMutationProline at position 104 changed to a LeucinePromoterCMVAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SM
Plasmid#136578PurposeDox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMycDepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa2 AS
Plasmid#69827PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only