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Plasmid#181859PurposePart of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.DepositorInsertcp-PKN-mRuby2-GFP(1-0)
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJA3
Plasmid#19520DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)
UseTagsV5ExpressionMammalianMutationF193L based on NP_005138.3PromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJB1
Plasmid#19522DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Caveolin-3
Plasmid#68396PurposeFluorescently tagged Caveolin-3 for expression in mammalian cellsDepositorInsertCav3 (Cav3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDHblast MCSNard OST-LMNA
Plasmid#22661DepositorInsertLamin A (LMNA Human)
UseLentiviralTagsOnestrep TagExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-ABE(7.10)
Plasmid#108387PurposeMammalian expression vector for xCas9(3.6)-ABEDepositorInsertxCas9(3.6)-ABE(7.10)
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF023_His_hsDDA1
Plasmid#174480PurposeExpresses DDA1 in insect cellsDepositorInsertDDA1 (DDA1 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-LckAPEX-P2A-EGFP
Plasmid#182826PurposeCre-dependent expression of membrane localized APEXDepositorInsertLckAPEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab1a S25N
Plasmid#46777Purposeexpression of S25N Rab1a in mammalian cellsDepositorInsertRab1a S25N mutant (RAB1A Human)
UseTagsmycExpressionMammalianMutationS25N (dominant negative)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_12xTEAD_luc2
Plasmid#173004Purposefirefly luciferase reporter vector with 12x clustered TEAD binding sitesDepositorInsert12xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Plasmid#137830PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
UseTags3x FLAGExpressionMammalianMutationMutated Leucine 71 to Lysine in CTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-VSVG
Plasmid#80606PurposeMammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the proteinDepositorInsertVSV-G
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HseIF4AI_AC
Plasmid#148438PurposeMammalian Expression of HseIF4AIDepositorInsertHseIF4AI (EIF4A1 Human)
UseTagsExpressionMammalianMutation3 silent mutations relative to NM_001416.2PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-Tpr
Plasmid#60882PurposeExpresses Flag-Tagged nucleoporin Tpr in Mammalian CellsDepositorInsertTpr (TPR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
px330 NIPBL gRNA
Plasmid#165590PurposeInsertion of NIPBL degronDepositorInsertNIPBL (Nipbl Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB2b
Plasmid#19496DepositorInsertDNAJB2b (DNAJB2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTwt full-length
Plasmid#109161PurposeEncodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length REST/NRSF (REST Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-NES-YFP-STOP-L2
Plasmid#127358Purposegateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only