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Plasmid#200783PurposePnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorinDepositorInsertins-22 (ins-22 Nematode)
UseTagspHiuorinExpressionWormMutationPromoterPnpr-4Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS1517
Plasmid#205980PurposeFLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP11 CS puroR
Plasmid#46750PurposeRetroviral vector that expresses catalytically inactive form of HA-tagged human USP11DepositorInsertUSP11 (USP11 Human)
UseRetroviralTagsHAExpressionMammalianMutationC318S--catalytically inactivePromoterCMVAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sox2-CreERT2
Plasmid#135618PurposeDonor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-CreERT2ExpressionMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS3Clux
Plasmid#55172Purposelux-reporter vector, integration at sacA, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
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p2T-cmv-SpCas9-TadCBEd-BlastR
Plasmid#193848PurposeExpress TadCBEd in mammalian cells with BlastR selection, Tol2 integrationDepositorInsertTadCBEd
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9(1.1)-2×sgRNA (empty, donor)
Plasmid#80768PurposeAll-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
UseTags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1-gs4_EF1a-Cas9-2A-GFP
Plasmid#118414PurposeCRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locusDepositorInsertgRNA AAVS1 (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:GFPNLS-SV40pA
Plasmid#155123PurposeTol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:GFPNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUASM1-E1bAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_puromycin
Plasmid#118595PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
Plasmid#155125PurposeTol2 vector containing Cre-inducible QFGal4-switchDepositorInsertQFGal4-switch
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoterUbiAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXNPH3
Plasmid#110098PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage siteDepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS1518
Plasmid#205981PurposeFLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1514
Plasmid#205977PurposeFLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas8/Cse1 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1515
Plasmid#205978PurposeFLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas11/Cse2 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1516
Plasmid#205979PurposeFLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas7 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT363
Plasmid#65858PurposeThe gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.DepositorInsertnahG
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Nkx2-1/Puro
Plasmid#31271DepositorInsertNkx2-1* (Nkx2-1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only