We narrowed to 3,761 results for: golden gate

1. SP_A4

   Plasmid

   #228148

   Purpose

   Plasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_Q6NUI6_CHADL_HUMAN:0-30
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SP_A5

   Plasmid

   #228149

   Purpose

   Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_O14793_GDF8_HUMAN:0-18
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKEK3236

   Plasmid

   #234108

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   sfCherry2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E117Q/T127I/G219A

   Promoter

   pJ23100-BsaI

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKEK3221

   Plasmid

   #234100

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   sfcherry 2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E117Q/T127I/G219A

   Promoter

   pJ23100-BsaI

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKEK3237

   Plasmid

   #234091

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   sfGFP
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   S30R/Y39N/N105T/Y145F/I171V/A206V

   Promoter

   pJ23100-BsaI

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKEK3397

   Plasmid

   #234081

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   FuGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available Since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKEK3224

   Plasmid

   #234086

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   FuGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available Since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKEK3197

   Plasmid

   #234095

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   YukonOFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKEK3199

   Plasmid

   #234097

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   tspurple
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKEK3200

    Plasmid

    #234098

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    fuGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKEK3232

    Plasmid

    #234104

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    tsPurple
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKEK3233

    Plasmid

    #234105

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    YukonOFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKEK3400

    Plasmid

    #234084

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLPP4

    Plasmid

    #209966

    Purpose

    Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P40)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLP-EV

    Plasmid

    #209973

    Purpose

    Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    CDS (mock)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLP-sfgfp

    Plasmid

    #209994

    Purpose

    Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLPT1

    Plasmid

    #209995

    Purpose

    Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    Terminator (TB1006)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLPS1

    Plasmid

    #209992

    Purpose

    Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    RBS (RB0034)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIS001

    Plasmid

    #194012

    Purpose

    This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

    Depositor

    Timothy Whitehead

    Article

    Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

    Insert

    ROSA-TRE-C16V5-G-pA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEM-T Easy-AtpU6-29

    Plasmid

    #160219

    Purpose

    AtpU6-29 Golden Gate level 0 piece to express gRNAs

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtU6-29 promoter
    

    Use

    Golden gate level 0 piece to express grnas

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge