Addgene

We narrowed to 3,013 results for: actin

Showing: 841 - 860 of 3013 results

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pFX-Lifeact-mCherry

Plasmid

#175822

Purpose

Express mCherry with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMB

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBA-mKeima-PIM

Plasmid

#174650

Purpose

Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

xXFKBP homo-mKeima-2x FKBP homo-2x FKBP

Use

Tags

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

Chicken beta-actin

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hEZH2

Plasmid

#112543

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Zhou et al Mol Ther. 2014 Feb;22(2):378-389. doi: 1

Insert

EZH2 (EZH2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-POLArISact

Plasmid

#164970

Purpose

T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1e

Plasmid

#134905

Purpose

Expression of rat Myo 1e (Myr 3) in mammalian cells.

Depositor

Martin Bähler

Article

Stoffler et al J Cell Biol. 1995 May;129(3):819-30. doi

Insert

Myo 1e (rat) with 3' UTR (Myo1e Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-H-Ras_wt

Plasmid

#39509

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Use

Eukaryotic

Tags

HA

Expression

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Plasmid

#173861

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-iRFP

Plasmid

#175823

Purpose

Express iRFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-Venus

Plasmid

#174478

Purpose

Express Venus with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV1_1-1009

Plasmid

#129471

Purpose

Mammalian expression of the N-terminus + central domain of bovine SV1

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

Supervillin (SVIL Bovine)

Use

Tags

Expression

Mammalian

Mutation

contains amino acids 1-1009

Promoter

CMV

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-H-Ras_V12

Plasmid

#39510

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Eukaryotic

Tags

Expression

Mutation

G12V

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pBa.Halo-Kif13B tail 442-1843

Plasmid

#162721

Purpose

Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Kif 13B (Kif13b Mouse)

Use

Tags

Expression

Mammalian

Mutation

WT with deletion of the 441 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP TEM4 DEL NTERM

Plasmid

#58894

Purpose

Expresses GFP tagged TEM4 with N terminus deleted

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deleted N Terminus up to aa 1002

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN beta delta C term

Plasmid

#40931

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C terminus deleted

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBABE-puroGFP-Eps8WT

Plasmid

#74956

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N54

Plasmid

#40934

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-54

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N112

Plasmid

#40935

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-112

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N173

Plasmid

#40936

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-173

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
EYFP-Parkin

Plasmid

#210292

Purpose

mitophagy marker

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-Parkin

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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