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Showing: 8561 - 8580 of 54832 results

  5. pDA2ScIfm

    Plasmid
    #193786
    Purpose
    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTATAA synthetic promoter carrying binding sites…
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193786-plasmid-map-sequence-id-389837

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  7. pTA1cI-T3

    Plasmid
    #193789
    Purpose
    Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Truncated version of Lambda CI, named T3, where the first two codons are deleted
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193789-plasmid-map-sequence-id-389812

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  9. pGWT35S-AtOEP7(1-50aa)-VC155

    Plasmid
    #194046
    Purpose
    Transient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)
    Depositor
    Yutaka Kodama
    Article
    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-
    Insert
    VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
    Use
    Tags
    AtOEP7 1–50aa
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    194046-plasmid-map-sequence-id-382264

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  10. pGWT35S-AtOEP7(1-50aa)-VN154

    Plasmid
    #194045
    Purpose
    Transient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)
    Depositor
    Yutaka Kodama
    Article
    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-
    Insert
    VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
    Use
    Tags
    AtOEP7 1–50aa
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    194045-plasmid-map-sequence-id-382260

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  11. pYPQ265K

    Plasmid
    #173997
    Purpose
    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    UNG-rAPOBEC1(R33A)-zCas9(D10A)
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    R33A in rAPOBEC1 and D10A in zCas9
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    173997-plasmid-map-sequence-id-343409

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  13. pNB_EA_R1CiM_4EGFP_R3EGFPi2

    Plasmid
    #185968
    Purpose
    Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.
    Depositor
    Sangram Bagh
    Article
    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-
    Insert
    Molecular IMPLY gate
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    185968-plasmid-map-sequence-id-370628

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  15. pNB_EA_R3tdTomatoi2

    Plasmid
    #185969
    Purpose
    Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.
    Depositor
    Sangram Bagh
    Article
    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-
    Insert
    Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PR
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185969-plasmid-map-sequence-id-370632

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Showing: 8561 - 8580 of 54832 results