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Plasmid#191011PurposeExpresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)DepositorInsertMicrotubule-associated serine/threonine kinase like (MASTL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9_sgRNA_0
Plasmid#70763Purposeexpresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicatingDepositorInsertsU6 promoter
cas9
tnos terminator
UseSelf-replicating in ustilago maydis, conferring c…TagsN-terminal NLS, C-terminal HA-tag +NLSExpressionMutationStreptococcus pyogenes gene codon-optimized for U…PromoterU6 from Ustilago maydis and synthetic otef promot…Available sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
UseTagsExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Olig1 [N149/25.1R]
Plasmid#114540PurposeMammalian Expression Plasmid of anti-Olig1 (Mouse). Derived from hybridoma N149/25.1.DepositorInsertanti-Olig1 (Mus musculus) recombinant mouse monoclonal antibody (Olig1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
UseTagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-miRFP-MAP4-MTBD
Plasmid#171487PurposeT7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNADepositorInsertMAP4-MTBD (Map4 Mouse)
UsePgehmeTagsmiRFPExpressionMutationAminoacids 658-1126PromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCA.66/2272
Plasmid#22733DepositorInsertLox66-pU(delta)TK-EM7Neo-Lox2272
UseCre/Lox; Bac recombineeringTagsExpressionMutationThis vector was designed for assembling gene targ…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Flrt2-myc
Plasmid#72192PurposeExpress full-length Flrt2with a C-terminal myc tagDepositorInsertFlrt2 (Flrt2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EPOP
Plasmid#125170PurposeExpresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertEPOP (EPOP Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-C236S-pEYFP_C1
Plasmid#85767Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236SDepositorInsertUSPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
UseTagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514), catalytic inactiv…PromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cLYN268
Plasmid#188259PurposePlasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcLYN268 (LYN Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLRC1-NEK10p:NEK10-3XFLAG
Plasmid#137030PurposeLentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoterDepositorInsertNIMA-like kinase 10 (NEK10 Human)
UseLentiviralTags3XFLAGExpressionMutationPromoterhuman NEK10 endogenous promoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-MOG [L94/54]
Plasmid#190296PurposeMammalian Expression Plasmid of anti-MOG (Rat). Derived from hybridoma L94/54.DepositorInsertanti-MOG (Rattus norvegicus) recombinant Mouse monoclonal antibody (Mog Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 KSR2
Plasmid#25968DepositorInsertKinase suppressor of Ras 2 (Ksr2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-KRAS-G12V
Plasmid#129091PurposeKI KRAS G12V mutation into human KRAS locusDepositorInsertKRAS(G12V) (KRAS Human)
UseHomologous recombination donor plasmidTagsExpressionMutationG12VPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLM-AMA15.0 Cas9
Plasmid#138944PurposeFungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markersDepositorInsertp40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
UseCRISPR and Synthetic Biology; Ama1 autonomously r…TagsNLSExpressionMutationPromoterp40S (AN0465) Aspergillus nidulansAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-Twin-Strep
Plasmid#202528PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Flrt1-myc
Plasmid#72191PurposeExpress full-length Flrt1 with a C-terminal myc tagDepositorInsertFlrt1 (Flrt1 Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-Nb127D01-GFP
Plasmid#171577PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembraneDepositorInsertCD8-Nb127D01-GFP (CXCR2 Human)
UseTagsCD8 and GFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pan-Neurofascin scFv [L11A/41]
Plasmid#206722PurposeMammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.DepositorInsertPan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only