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Plasmid#213318PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR1D (HTR1D Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188774PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-QKI-5 [N195A/16R]
Plasmid#114481PurposeMammalian Expression Plasmid of anti-QKI-5 (Human). Derived from hybridoma N195A/16.DepositorInsertanti-QKI-5 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode
Plasmid#170240PurposeBarcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsExpressionMutationS218D, S222DPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1
Plasmid#112261PurposeDonor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.DepositorInsertspCas9-p300 (EP300 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.N
Plasmid#127851Purposenon-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.N VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188772PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterCMVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
GIV-WT - FLAG
Plasmid#65947PurposeExpression GIV/Girdin in Mammalian cellDepositorInsertGIV/Girdin (CCDC88A Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
HTR1B-DuET
Plasmid#213317PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR1B (HTR1B Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CALCRb-DuET
Plasmid#213193PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCALCRb (CALCRL Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG Chtop-exon minimal CMV pCDNA5
Plasmid#212080PurposeLR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cellsDepositorInsertChtop-exon (Chtop Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLV-gag-hMLH1dn
Plasmid#195513PurposeMMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.DepositorInserthMLH1dn (MLH1 Human)
UseVirus like particleTagsFLAG, NES, and Protease cleavate siteExpressionMammalianMutationHuman MLH1 del754-756PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-2t
Plasmid#159752PurposeCRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.DepositorArticleInsertsgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-P2A-EGFP
Plasmid#188898PurposeHuman lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9
UseLentiviralTagsHA-2xNLS and P2A-GFPExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTUU
Plasmid#159749PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Shank3 [N69/46R]
Plasmid#140078PurposeMammalian Expression Plasmid of anti-Shank3 (Rat). Derived from hybridoma N69/46.DepositorInsertanti-Shank3 (Rat) recombinant mouse monoclonal antibody (Shank3 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.kc1-HSD17B10
Plasmid#164076PurposeHuman codon optimized HSD17B10. Expression is controlled by doxycycline.DepositorInsertHSD17B10 (HSD17B10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-Olig1 [N149/25.1R]
Plasmid#114540PurposeMammalian Expression Plasmid of anti-Olig1 (Mouse). Derived from hybridoma N149/25.1.DepositorInsertanti-Olig1 (Mus musculus) recombinant mouse monoclonal antibody (Olig1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mNeonGreen-CCR5TC
Plasmid#135534PurposeEncodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmNeonGreenExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mEos3.2-CCR5TC
Plasmid#135532PurposeEncodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmEos3.2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only