Addgene

We narrowed to 23,086 results for: GFP

Showing: 8481 - 8500 of 23086 results

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pHAGE-eGFP-OPTN S513A

Plasmid

#175764

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

Depositor

Wade Harper

Article

Eapen et al Elife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Tags

Expression

Mutation

S513A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-attB-PUb-GFP

Plasmid

#183911

Purpose

attB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

Aedes aegypti PUb

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS956 IBB-GFP-mCherry3E

Plasmid

#107859

Purpose

Flow cytometry-compatible reporter for TDP43 function in splicing

Depositor

Rajat Rohatgi

Insert

Synthetic splicing reporter (TARDBP Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE GFP-Ago2 S387E

Plasmid

#60655

Purpose

GFP-Ago2 S387E mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

S387E

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CsChR-GFP

Plasmid

#58841

Purpose

AAV expression of CsChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

Plasmid

#52478

Purpose

Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
nuclear mTagBFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBSU6-shTim23/CMV-eGFP

Plasmid

#89795

Purpose

Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tim23 (Timm23 Mouse)

Use

Tags

eGFP under separate promoter

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4E-F47AI79A_Z

Plasmid

#148136

Purpose

Mammalian Expression of HseIF4E-F47AI79A

Depositor

Elisa Izaurralde

Insert

HseIF4E-F47AI79A (EIF4E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-N95 (for GFP)

Plasmid

#130716

Purpose

Plasmid encoding the bacterial expression of LAMA-N95 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-N95

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB3-MUT

Plasmid

#194565

Purpose

Mammalian Expression of mEGFP-HMGB3 Mutant (K161Ifs*55) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB3 (HMGB3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K161Ifs*55

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHBS1415 GFP-TDP43 VLIM-S

Plasmid

#133323

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

ALL VLIM in the TDP43 IDRs mutated to S

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP MPS1 K-R

Plasmid

#59819

Purpose

Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

Insert

Mps1 (TTK Human)

Use

Tags

EmGFP

Expression

Mammalian

Mutation

KD, catalytically inactive, D664A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pXGFPC-5 Pxyl-dcas9 (Spy)

Plasmid

#133316

Purpose

expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus pyogenes)

Use

CRISPR

Tags

Expression

Mutation

Promoter

xylose

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-C

Plasmid

#174737

Purpose

Overexpresion

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-GFPIvy-SpHis5

Plasmid

#60783

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPIvy

Use

Yeast integrating

Tags

Expression

Mutation

S30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1-Poly(A)

Plasmid

#65559

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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KP201: pMVP (L5-L4) eGFP-P2A

Plasmid

#121708

Purpose

pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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BAS286 C terminal SF-GFP hygBR

Plasmid

#74081

Purpose

S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

super-folder GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
M71-IRES-tauGFP LTNL TV

Plasmid

#15639

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M71-IRES-tauGFP LTNL TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only