Addgene

We narrowed to 32,871 results for: tes

Showing: 8461 - 8480 of 32871 results

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pPotef-cdc3CB16-NatR

Plasmid

#86465

Purpose

Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

Depositor

Michael Feldbrügge

Article

Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

Insert

Cdc3

Use

Ustilago maydis expression

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Potef

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-cMyc T58A

Plasmid

#20075

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only
pSH231-EF1-RFP-HYGRO

Plasmid

#115145

Purpose

Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-CAND1

Plasmid

#19949

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pLL3.7-K122-TEAD-p-H2B-mCherry

Plasmid

#139648

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

IRES-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3A T32A

Plasmid

#8348

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T32A

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
pvimentin-emiRFP670

Plasmid

#136572

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP670

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-Puro

Plasmid

#115143

Purpose

Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Puromycin

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-CUL4A delta N52

Plasmid

#19952

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Missing 52 residues from N terminal CUL4A.

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3A S253A

Plasmid

#8349

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S253A

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
SaLgCP

Plasmid

#164562

Purpose

Puro

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Flag-NLS-SaCas9-P2A-PuroR

Expression

Mammalian

Mutation

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFPB5A

Plasmid

#83556

Purpose

Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

GAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4A-2K-NS4B

Plasmid

#120828

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4A-2K-NS4B ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-PKCg-mEGFP-N1

Plasmid

#112270

Purpose

IDOCKSgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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DAXX-myc-his

Plasmid

#1852

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

Plasmid

#78509

Purpose

Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD45R0Ex (PTPRC Human)

Use

Tags

His10 and SNAPf

Expression

Insect

Mutation

Promoter

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP-FLAG)

Plasmid

#102424

Purpose

Dual tagged RILP for expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Tet-Responsive

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMIEG3-BCL6

Plasmid

#40339

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

optimized translation initiation codon

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only