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Plasmid#72591PurposePlasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A C-Term
Plasmid#69795PurposePebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble C-Term domain (Drosophila guanine nucleotide exchange factor)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationJust the C-Term domain of Pebble (pbl)Promoterhsp70 promoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)1x
Plasmid#112055PurposeACTB mRNA tagged with 1 copy of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation1 copy of Riboglow tag (variant A) after stop cod…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)4x
Plasmid#112058PurposeACTB mRNA tagged with 4 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation4 copies of Riboglow tag after stop codonPromoterCMVAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-ATF4 mScarlet NLS
Plasmid#115969PurposeER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-IDepositorInsertactivating transcription factor 4 (ATF4 Human, Synthetic)
UseLentiviralTagsHA tag, c-myc NLS, and mScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST p38KTRClover
Plasmid#59152PurposeLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mRuby2-CCR5TC
Plasmid#135531PurposeEncodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph15-SNAPf-CCR5TC
Plasmid#135536PurposeEncodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
UseTagsSNAPfExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-Mut-T-Luc
Plasmid#60490PurposeContains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988DepositorInsertHMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
UseLuciferaseTagsLuciferase reporterExpressionMammalianMutationFour point mutations (G->C or A->C) in the …PromoterPartial HMG-CoA synthase promoter (-324/-225) fus…Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
CCR7-DuET
Plasmid#213203PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR7 (CCR7 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-uvsX-H6
Plasmid#163913PurposeExpresses uvsX for bacterial expression and affinity purificationDepositorInsertuvsX
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CX3C1-DuET
Plasmid#213217PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCX3C1 (CX3CR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR2-DuET
Plasmid#213198PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR2 (CCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
GABBR1-DuET
Plasmid#213247PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGABBR1 (GABBR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EDNRB-DuET
Plasmid#213233PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertEDNRB (EDNRB Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
F2R-DuET
Plasmid#213234PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertF2R (F2R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EDNRA-DuET
Plasmid#213232PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertEDNRA (EDNRA Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR8-DuET
Plasmid#213204PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR8 (CCR8 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only