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Plasmid#149637Purposesuicide vector for knock in into the B. burgdorferi chromosomeDepositorInsertdCas9, lacI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2SC
Plasmid#59382PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2SK
Plasmid#59383PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.DepositorInsertTT-ISceI-kan
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAT77
Plasmid#204295PurposepJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pRSETB
Plasmid#109484Purposebacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
UseTagsN-terminal histidine tag in vectorExpressionBacterialMutationPromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAT21
Plasmid#204309PurposeFor generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthpartsDepositorInsertie1-GFPn
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT25
Plasmid#204310PurposeFor generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.DepositorInsertie1-GFPc
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24-1A(sfGFP)
Plasmid#126971PurposeLevel 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-surrogate-reporter
Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1DepositorInsertattP
UseBxb1TagsExpressionMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEN_TRE_GFP
Plasmid#25767PurposeEntry vector with TRE promoter driving GFP expression.DepositorTypeEmpty backboneUseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX128·gRNA
Plasmid#187600PurposeTemplate for amplification of gRNA with Cas6 recognition siteDepositorInsertgRNA template
UseTagsExpressionMutationPromoterno promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-05
Plasmid#119984PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
mSA-phi29
Plasmid#210815Purposein vitro transcription of mRNADepositorInsertmSA-Phi29
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT27
Plasmid#204308PurposeFor generating targeting inversions. C-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPc
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT18
Plasmid#204307PurposeFor generating targeting inversions. N-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPn
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHO-UAS-mScarlet-I
Plasmid#183147PurposeYeat two-hybrid fluorecent reporter for genomic integrationDepositorInsertmScarlet-I
UseTagsExpressionMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOPO GFP
Plasmid#68488PurposeTOPO construct expressing GFP for generation of GMAP-compatible geneDepositorInsertGreen Fluorescent Protein
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEH373 dTomato 3x high affinity MS2 loops
Plasmid#168221PurposeMammalian expression of dTomato with 3 high affinity MS2 loopsDepositorInsertdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only