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Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag
Plasmid#179719PurposeLentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag
Plasmid#179721PurposeLentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag
Plasmid#179723PurposeLentiviral expression of lysosome-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) U6-SYT7 sgRNA 777 HaloTag
Plasmid#179724PurposeU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITIDepositorInsertsgRNA and HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn
Plasmid#174824PurposeMammalian expression of human MLH1dn (codon optimized)DepositorInserthuman MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDV06.2c_DART-VADAR_iRFP720-sensor
Plasmid#200410PurposepDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2DepositorInsertClosed-loop DART VADAR sensor detecting iRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200403PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_18xTEAD_luc2
Plasmid#173003Purposefirefly luciferase reporter vector with 18x clustered TEAD binding sitesDepositorInsert18xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag YAP2 5SA
Plasmid#27371DepositorInsertYAP2 (YAP1 Human)
UseTagsflagExpressionMammalianMutationSerines at positions 61, 109, 127, 128, 131, 163,…PromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mMLH1dn
Plasmid#174825PurposeMammalian expression of mouse MLH1dn (codon optimized)DepositorInsertmouse MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 758-760PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-mRxxFP1(AfeI)-iP-A
Plasmid#204625PurposeCloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianMutationPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA/EF1a-mCherry
Plasmid#114199PurposeLentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoterDepositorInsertsgRNA scaffold
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only