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Plasmid#196281PurposeGateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell linesDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCW57.1_V5_BirA*_FLAG_Nt
Plasmid#194070Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-BirA(R118G)-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_BirA*_FLAG_Ct
Plasmid#194071Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-BirA(R118G)-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-NatMx
Plasmid#158563PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-LIC+
Plasmid#62312PurposeE. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage siteDepositorTypeEmpty backboneUseTags6xHis and thrombin protease cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-1x-ccdB-6stop
Plasmid#203519PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mScarlet-I-stop
Plasmid#186393PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREP4xN_ccdB
Plasmid#79022PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pREP82xN_ccdB
Plasmid#79026PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP81xN_ccdB
Plasmid#79025PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBL6951
Plasmid#217373PurposesfGFP reporter for Bsu pbuE* 2AP riboswitchDepositorInsertPca rhtB riboswitch sfGFP reporter
UseTagsExpressionMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL730
Plasmid#217374PurposesfGFP reporter for Bsu yxjA 2AP riboswitchDepositorInsertyxjA riboswitch sfGFP reporter
UseTagsExpressionMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7368
Plasmid#217375PurposesfGFP reporter for Bce crcB fluoride riboswitchDepositorInsertBce crcB riboswitch sfGFP reporter
UseTagsExpressionMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_nahR-PSalTTC
Plasmid#204074PurposeType 0 promoter partDepositorInsertnahR repressor and PSalTTC sodium salicylate-inducible promoter
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-T_his-min-S
Plasmid#204123PurposeType 0 terminator partDepositorInserthis-min-S strong terminator
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-T_BBa-B1006-U10
Plasmid#204122PurposeType 0 terminator partDepositorInsertBBa-B1006-U10 strong terminator
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD22
Plasmid#204104PurposeType 0 tag-compatible RBS partDepositorInsertMCD22 weak RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-TagR_MCD8
Plasmid#204105PurposeType 0 tag-compatible RBS partDepositorInsertMCD8 weak RBS for assembly with tag part
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only