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Addgene
Showing: 8361 - 8380 of 20573 results
  1. MultiMate-PE2 HEK3

    Plasmid
    #206274
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
    Depositor
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production, cre-a…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pMMACE DEST CMV PE2

    Plasmid
    #206276
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Insert
    PE2
    Use
    CRISPR; Recombinant baculovirus production, multi…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Insert
    Kanamycin cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Availability
    Academic Institutions and Nonprofits only
  5. pZNF764-donor

    Plasmid
    #112298
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF764 with GFP
    Depositor
    Insert
    ZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:30555233:G>A, hg38:16:30555234:C>G,…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pZNF75A-donor

    Plasmid
    #112307
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF75A with GFP
    Depositor
    Insert
    ZNF75A homology arms flanking EGFP-IRES-Neo cassette (ZNF75A Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:3317462:A>T, hg38:16:3317715:C>T, h…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pZNF84-donor

    Plasmid
    #112330
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF84 with GFP
    Depositor
    Insert
    ZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs623100, hg38:12:133058872:T>C, hg38:12:13305…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pZBTB26-donor

    Plasmid
    #112356
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB26 with GFP
    Depositor
    Insert
    ZBTB26 homology arms flanking EGFP-IRES-Neo cassette (ZBTB26 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:9:122919395:A>G, hg38:9:122919458:T>G,…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pELF4-donor

    Plasmid
    #112371
    Purpose
    CRISPR donor plasmid to tag human transcription factor ELF4 with GFP
    Depositor
    Insert
    ELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pZNF41-donor

    Plasmid
    #112375
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF41 with GFP
    Depositor
    Insert
    ZNF41 homology arms flanking EGFP-IRES-Neo cassette (ZNF41 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:X:47447496:G>T, hg38:X:47448269:C>A, h…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pZNF215-donor

    Plasmid
    #113804
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF215 with GFP
    Depositor
    Insert
    ZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs2239733, rs2239732, hg38:11:6956348:T>G, rs1…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pCSDC2-donor

    Plasmid
    #113813
    Purpose
    CRISPR donor plasmid to tag human transcription factor CSDC2 with GFP
    Depositor
    Insert
    CSDC2 homology arms flanking EGFP-IRES-Neo cassette (CSDC2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 8361 - 8380 of 20573 results