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Plasmid#177181PurposepegRNA cloning plasmid with BFP markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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lentiCRISPR - AAVS1 sgRNA
Plasmid#70661PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backboneDepositorInsertsgRNA against AAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-RLuc-T2A-PuroR
Plasmid#102320Purposeexpression of r-luciferaseDepositorInsertRenilla luciferase
UseLentiviralTagsExpressionMutationPromoterUbcAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Del 4)
Plasmid#16604DepositorInsertc-myc promoter (Del 4) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationDel-4 (PvuII->PvuII). The first PvuII site is …PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
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V247 pCEP 4HA B56gamma1
Plasmid#14534DepositorInsertPPP2R5C B56 gamma1 regulatory subunit of PP2A (PPP2R5C Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA+WT GADD45
Plasmid#24929DepositorInsertGADD45 (GADD45A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TrxRFP2
Plasmid#172322Purposean improved fluorescent biosensor for thioredoxin redoxDepositorInsertTrxRFP2, an improved version from TrxRFP1
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6899 PHAGE-P CMVT N-HA-hE6AP II C843A
Plasmid#37604DepositorInsertE6AP II C843A (UBE3A Human)
UseTagsHAExpressionMammalianMutationC843APromoterCMVtAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pFF-41.5-mCherry
Plasmid#192863PurposepMUT2 based vector for sensing oxygen in E. coli Nissle 1917DepositorInsertOxygen inducible promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG2-wPRE-pA
Plasmid#60489PurposeeGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)DepositorInsertIRES-eGFP; wPRE; BGH-pA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST-PSP-hTG
Plasmid#85470Purposebacterial expression of human tissue transglutaminaseDepositorInserthTG2 (TGM2 Human)
UseTagsGST and PSPExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-TRP1
Plasmid#41599DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags3xHA and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
UseTagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…PromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO-mito-mEm
Plasmid#185596PurposeLentiviral construct for expression of mEmerald targeted to mitochondriaDepositorInsertmito-mEm
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.E2Crimson
Plasmid#100894PurposeLentiviral gRNA deliveryDepositorInsertE2Crimson
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC-GoldyTALEN
Plasmid#38143Purposedestination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoterDepositorInsertGoldyTALEN
UseTagsAcV5 and FokI homodimerExpressionMammalianMutationTruncate at N and C terminousPromoterminiCaggsAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMAL498
Plasmid#169154PurposeEncodes OptoQ-INVRT4DepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only