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Plasmid#184567PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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EF1a_KLF4_P2A_Hygro_Barcode
Plasmid#120455PurposeBarcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9-T2A-GFP
Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-K263E
Plasmid#133758PurposeLentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged lysine 263 to glutamate.PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A315T
Plasmid#133755PurposeLentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged alanine 315 to threoninePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-P112H
Plasmid#133760PurposeLentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged proline 112 to histidine.PromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-M337V
Plasmid#133759PurposeLentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged methionine 337 to valinePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-Q331K
Plasmid#133761PurposeLentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged glutamine 331 to lysine.PromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Beta-Globin-PTC39
Plasmid#130701PurposeExpresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)DepositorInsertBeta Globin (HBB Human)
UseTagsExpressionMammalianMutationPTC39PromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-mAgo2-EGFP
Plasmid#209935PurposeLentiviral expression of mouse Argonaute-2 in mammalian cellsDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5c-Myc
Plasmid#186616PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5c (KIF5C Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A90V
Plasmid#133754PurposeLentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged alanine 90 to valinePromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-D169G
Plasmid#133757PurposeLentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged aspartate 169 to glycine.PromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-deGFP
Plasmid#184840PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E2P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-basEgfp
Plasmid#90144Purposecontains dll4 enhancer 2 sequence and a basal promoter driving expression of eGFPDepositorInsertdll4E2basEgfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-TGFbeta receptor II wt
Plasmid#11766DepositorInsertTGFbeta receptor II (TGFBR2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tet-O-FUW-Olig2
Plasmid#45844DepositorInsertOlig2 (Olig2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromotertet-ONAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only