-
Plasmid#167638PurposeExpress FepA I14C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA I14C
UseTagsExpressionBacterialMutationfepA with I14C mutation (Mutation is I36C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1383
Plasmid#167639PurposeExpress FepA V15C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V15C
UseTagsExpressionBacterialMutationfepA with V15C mutation (Mutation is V37C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1384
Plasmid#167641PurposeExpress FepA V16C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V16C
UseTagsExpressionBacterialMutationfepA with V16C mutation (Mutation is V38C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1416
Plasmid#167643PurposeExpress FepA T17C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T17C
UseTagsExpressionBacterialMutationfepA with T17C mutation (Mutation is T39C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1583
Plasmid#167645PurposeExpress FepA D34C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D34C
UseTagsExpressionBacterialMutationfepA with D34C mutation (Mutation is D56C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1382
Plasmid#167647PurposeExpress FepA G54C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA G54C
UseTagsExpressionBacterialMutationfepA with G54C mutation (Mutation is G76C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP718
Plasmid#167636PurposeExpress FepA T13C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T13C
UseTagsExpressionBacterialMutationfepA with T13C mutation (Mutation is T35C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1410
Plasmid#167634PurposeExpress FepA D12C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D12C
UseTagsExpressionBacterialMutationFepA with D12C mutation. (Numbering based on star…PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
24 pCOLI_A_N_STREP_C_FKBP
Plasmid#160260PurposeN-term 2xStrepII 3C cleavable, C-term FKBP Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepII and FKBPExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
20 pCOLI_A_N-His_C_STREP
Plasmid#160162PurposeN-term 6xHis 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepII and 6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP89b CvTA
Plasmid#141204PurposeChromobacterium violaceum transaminase with TEV cleaving site after the N-terminal Histag.DepositorInsertCvTA
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
psubCMV-WPRE-IL3SP-mVEGF-D-dNdC
Plasmid#119229PurposePlasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.DepositorInsertIL3SP-Vegfd-dNdC (Vegfd Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAT6
Plasmid#112594PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneUseTags6xHis-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAT5
Plasmid#112593PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneUseTags6xHis-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
STK10 (STK10A-c043)
Plasmid#110255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSTK10 (STK10 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-C195A
Plasmid#83408Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC195A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-C29A
Plasmid#83407Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC29A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-V28A
Plasmid#83406Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationV28A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mKOet-LaminB1-10
Plasmid#57928PurposeLocalization: Nuclear Envelope, Excitation: 548, Emission: 561DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmKOetExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PP-MAPK2 (3RP9)
Plasmid#32862PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK2
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acids 123-548 only, P421LPromoterAvailable sinceJan. 10, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits