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Plasmid#134990PurposedCas9-mediated activation of HOTTIP in mammalian cellsDepositorInsertHOTTIP (HOTTIP Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterU6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)‐NeoR
Plasmid#65526PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV Gsk3 sgRNA/GFP
Plasmid#112733PurposeGsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.DepositorInsertGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5
Plasmid#112840Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationFull length Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV KSR1 IRES GFP
Plasmid#25973DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-STAT3-linker-TagGFP2
Plasmid#121427PurposeWT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertSTAT3-linker-TagGFP2
UseTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GFP
Plasmid#39290DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPH3
Plasmid#19482DepositorInsertHSPH3 (HSPA4L Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
PITX3-2A-eGFP-PGK-Puro
Plasmid#31943DepositorInsertPITX3 (PITX3 Human)
UseTargeting donorTagseGFPExpressionMutationPromoter-Available sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
B3GALT5_pLENTI-CAG-IRES-GFP
Plasmid#177006PurposeMammalian lentiviral expression vector encoding B3GALT5DepositorInsertB3GALT5 (B3GALT5 Human)
UseLentiviralTagsExpressionMutationPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP/RFP-int
Plasmid#19818DepositorTypeEmpty backboneUseCre/Lox and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HtrA2-EGFP S306A
Plasmid#14122DepositorInsertHtrA2 (HTRA2 Human)
UseTagsEGFPExpressionMammalianMutationS306APromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP2AKAT7-linker-mAID-W
Plasmid#159294PurposeLentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoterDepositorInsertKAT7 (KAT7 Human)
UseLentiviralTagsmAID (auxin-inducible degron)-taggedExpressionMammalianMutationCRISPR sites mutated (gRNA ID. 5 and A10)Promoterhuman EF1a promoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA-mCherry
Plasmid#99876PurposeExpression of GFP-AURKA-mCherry for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFP and mCherryExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC+CKK
Plasmid#59041Purposefor baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cellsDepositorInsertCAMSAP3 CC+CKK domain (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
LoxP2-H2B-EGFP-V5-2A (SO107)
Plasmid#99617PurposeTo clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2ADepositorInsertH2B-EGFP-V5
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-eGFP
Plasmid#117770PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP (pJJF001)
Plasmid#173457PurposeC-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
Plasmid#122471PurposeDox-inducible GFP (N-terminal tag) SCAF4 expressionDepositorInsertSCAF4 (SCAF4 Human)
UseTagsGFPExpressionMammalianMutationSynonymous mutations conferring resistance to gRN…PromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only