Addgene

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p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-D-TOPO-kcnn1b

Plasmid

#164957

Purpose

Zebrafish kcnn1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1b (kcnn1b Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBS YBX1

Plasmid

#19217

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

YBX1 (YBX1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
XE131 XFz1-CS2P+

Plasmid

#16793

Depositor

Randall Moon

Article

Brown et al Dev Growth Differ. 2000 Aug. 42(4):347-5

Insert

XFz1-CS2P+ (fzd1 Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-AaCas12b(D977A)-2AeGFP

Plasmid

#121962

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

D977A

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGDP1 vph

Plasmid

#112893

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

vph

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iE

Plasmid

#83312

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iE

Use

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iE betwe…

Promoter

T5

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
SUN2delSUN

Plasmid

#130835

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of SUN domain

Promoter

CaMV 35S

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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alpha7-nAChR(scramble control)

Plasmid

#86649

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNA7 (control)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;clec14aCE1-basEgfp

Plasmid

#90140

Purpose

contains clec14a enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1basEgfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-deltaNLS1-EGFP

Plasmid

#22150

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 297-304

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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MCV-hVam6p-

Plasmid

#32059

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-hVam6p-

Use

Tags

Expression

Mutation

TG to GC mutations at 3280-3281 (nt) of full cons…

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-pol30(K127/164R)-3'

Plasmid

#99549

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K127R, K164R

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330)

Plasmid

#87738

Purpose

Bacterial expression of the VSL domain of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-279

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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hPlekhm1_pIRES puro Glue

Plasmid

#73453

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pQStrep2-PREI3

Plasmid

#31592

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

preimplantation protein 3 (MOB4 Human)

Use

Tags

His and StrepII-tag

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-N-Flag-8His

Plasmid

#157677

Purpose

N-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Flag-8His

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGDP3 vatD

Plasmid

#112885

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

vatD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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