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Plasmid#146065PurposeInsect Expression of DmMe31B_1-267DepositorInsertDmMe31B_1-267 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B_268-459_C
Plasmid#146066PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Sirius-KRasCT
Plasmid#175831PurposeExpress Sirius with plasma membrane-targeting sequence in mammalian cellsDepositorInsertSirius with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-SECFP-KRasCT
Plasmid#175832PurposeExpress SECFP with plasma membrane-targeting sequence in mammalian cellsDepositorInsertSECFP with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK1179
Plasmid#179296PurposeExpresses dCas9 fused to four DmrA domainsDepositorInsertdCas9-DmrA(x4)
UseTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterCAGAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Pigb-V5
Plasmid#175160PurposeLentiviral expression of mouse Pigb-V5DepositorInsertPigb (Pigb Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem209
Plasmid#175154PurposeLentiviral expression of V5-tagged mouse Tmem209DepositorInsertTmem209 (Tmem209 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mSpata33-PA
Plasmid#179129PurposeExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertspermatogenesis associated 33 (Spata33 Mouse)
UseTagsPAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hSPATA33-8xHIS-1D4
Plasmid#179133PurposeExpression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertspermatogenesis associated 33 (SPATA33 Human)
UseTags1D4 and 8XHISExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Zdhhc6-V5
Plasmid#175146PurposeLentiviral expression of mouse Zdhhc6-V5DepositorInsertZdhhc6 (Zdhhc6 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Ptdss2-V5
Plasmid#175143PurposeLentiviral expression of mouse Ptdss2-V5DepositorInsertPtdss2 (Ptdss2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-SspB-HaloTag-TIAM1-DHPH
Plasmid#176134PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-LLO-hPMEL
Plasmid#174607Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitopeDepositorInsertexpresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneUseTagsCmyc and HAExpressionYeastMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#2
Plasmid#163389PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#1
Plasmid#163388PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#3
Plasmid#163390PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSunS 1-335
Plasmid#171673PurposeSunS 1-335 expression in E. coliDepositorInsertGlycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_stgRNA-Puro
Plasmid#164124PurposeBackbone for lentiviral stgRNA library construction (library 1 and 2)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 TNos:Luc:5'UTR (GB1499)
Plasmid#160580PurposeReversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoterDepositorInsertLuc
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only