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Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP TFEB-sfGFP
Plasmid#135402PurposeExpresses TFEB-sfGFPDepositorInsertTFEB (TFEB Human)
UseRetroviralTagssfGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOCT4
Plasmid#20726DepositorInsertOCT4 (POU5F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
LeGO-mOrange2
Plasmid#85212PurposeLentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoterDepositorInsertmOrange2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT001
Plasmid#182711PurposeaTc inducible dCasRxDepositorInsertdCasRx
UseCRISPRTags2x-FLAGExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
VN-gamma Synuclein
Plasmid#89733Purposeexpresses human gamma Synuclein fused to the N-terminal part of VenusDepositorInsertSNCG (SNCG Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-LEXY (pDN122)
Plasmid#72655PurposeBlue light-inducible nuclear export of mCherryDepositorInsertNLS-mCherry-LEXY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab5
Plasmid#174454PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
UseTagssmall GTPase (Rab5)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
CFP-FKBP (CF)
Plasmid#20160DepositorInsertCFP-FKBP (FKBP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
UseTagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X7
Plasmid#182366PurposeExpresses Neq2X7 DNA polymeraseDepositorInsertNeq2X7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnCasPA-BEC
Plasmid#113349PurposeA cytidine deaminase-mediated base-editing plasmid in Pseudomonas speciesDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307
Plasmid#41392PurposeConstitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 WT
Plasmid#174447PurposeExpress GFP-tagged RNase H1 WT in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KEAP1_WT
Plasmid#81925PurposeGateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.DepositorInsertKEAP1 (KEAP1 Human)
UseGateway entry vectorTagsExpressionMutationC624SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_IDH1_p.R132H
Plasmid#81686PurposeGateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.DepositorInsertIDH1 (IDH1 Human)
UseGateway entry vectorTagsExpressionMutationR132HPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (RFP)
Plasmid#80923PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9TagsExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only