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Plasmid#217321PurposePurification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NHSTIIT-GG
Plasmid#215789PurposeCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-GST-TEV-GG
Plasmid#215795PurposeCloning of target gene with N terminal GST tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-MP-84-spCas9-U6-gRNA-tlock
Plasmid#206936PurposeExpression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.DepositorInsertspCas9
UseAAVTagsSV40 NLSExpressionMammalianMutationPromoterMP-84Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-mut2-dsRNAres_Q
Plasmid#147264PurposeInsect Expression of DmNot3-mut2-dsRNAresDepositorInsertDmNot3-mut2-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-F764A-dsRNAres_Q
Plasmid#147280PurposeInsect Expression of DmNot3-F764A-dsRNAresDepositorInsertDmNot3-F764A-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-F818A-dsRNAres_Q
Plasmid#147281PurposeInsect Expression of DmNot3-F818A-dsRNAresDepositorInsertDmNot3-F818A-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-mut1-dsRNAres_Q
Plasmid#147282PurposeInsect Expression of DmNot3-mut1-dsRNAresDepositorInsertDmNot3-mut1-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-mut2-dsRNAres_Q
Plasmid#147283PurposeInsect Expression of DmNot3-mut2-dsRNAresDepositorInsertDmNot3-mut2-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-F764A-dsRNAres_Q
Plasmid#147261PurposeInsect Expression of DmNot3-F764A-dsRNAresDepositorInsertDmNot3-F764A-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-mut1-dsRNAres_Q
Plasmid#147263PurposeInsect Expression of DmNot3-mut1-dsRNAresDepositorInsertDmNot3-mut1-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppie.001.301.W257A
Plasmid#137652PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppie (PPIE Human)
UseTagsGST-(His)6-thrombinExpressionBacterialMutation001-301-W257APromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.522.K45A.pc4
Plasmid#137648PurposeExpresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTagsNLS and myc-(His)6ExpressionMammalianMutation001-522-K45A-pc4PromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.522.Y32A.pc4
Plasmid#137646PurposeExpresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTagsNLS and myc-(His)6ExpressionMammalianMutation001-522-Y32A-pc4PromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q
Plasmid#147262PurposeInsect Expression of DmNot3-F818A-dsRNAresDepositorInsertDmNot3-F818A-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
C-BRPF3-8
Plasmid#166516PurposescFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
58 pLIB_SNAP
Plasmid#160307PurposeN-term 3C cleavable SNAP tag for insect cell expressionDepositorTypeEmpty backboneUseTags12xHis and SNAPExpressionInsectMutationPromoterpolyhydrinAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
38 pCOLI_K_ C_STREP
Plasmid#160266PurposeC-term 2xStrepII uncleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepIIExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
13 pCOLI_A_CBP
Plasmid#160155PurposeN-term CBP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTagsCBPExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
35 pCOLI_K_HA
Plasmid#160178PurposeN-term HA 3C cleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags3xHA and 6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only