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Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stableā¦TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-URA3-3'SEC13-GFP
Plasmid#25445DepositorInsertSec13-Green Fluorescent Protein
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TetR-NLS-VP16
Plasmid#103834PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInsertEGFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-gcry-eGFP
Plasmid#117767PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL FKBP 4K>R
Plasmid#202421PurposeExpression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsFKBP and GFPExpressionMutationK431/525/526/547RPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RIĪ±Ī(D/D+linker)-EGFP
Plasmid#181843PurposeGFP-tagged PKA RIĪ± subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.DepositorInsertRIĪ±-(D/D+linker)EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationDeletion of residues 12-112 in PKA RIĪ±.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP-FHA-SHLD2-m1
Plasmid#114122PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (m1 mutant) (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianMutationW489A, F494A, W495A mutations introducedPromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXJ40-GFP-iBox-PAK4cat
Plasmid#190179Purposeexpresses GFP-tagged iBox-PAK4catDepositorInsertGFP-iBox-PAK4cat
UseTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB (S133A)
Plasmid#137002PurposemEGFP-CREB donor for G-CREB with S133A mutationDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationChanges Serine 133 into AlaninePromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-YAP (S94A)
Plasmid#174173PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a geneā¦ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_8-eGFP
Plasmid#107411PurposeThis plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrC3_8Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b1-Poly(A)
Plasmid#65531PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locusDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-1xGFP-6xHis
Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-9A
Plasmid#114023PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorInserteGFP-HEC1 - 9A (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 9APromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP109 pEF1a-gRNA-GFP
Plasmid#100935PurposeGFP, puromycin and gRNA scaffold driven by EF1a promoterDepositorInsertgRNA scaffold
UseGrna scaffoldTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1KD:GFP
Plasmid#105645PurposeKinase-deleted Ikk1(alpha) expression in basal keratinocytesDepositorInserttp63:ikk1KD:GFP (chuk Zebrafish)
UseTagsGFPExpressionMammalianMutationp63 promoter + ikk1 (kinase domain deleted) + GFPPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG.3xHalo-GFP-SPC25
Plasmid#176206PurposeKinetochore anchor for TMP-Halo dimerization systemDepositorInsert3xHalo-GFP-SPC25
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-eGFP-P2A-PuroR-HA
Plasmid#163343PurposeRetroviral vector to express cytosolic GFP and an HA-tagged puromycin selection markerDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only