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Plasmid#110081PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pDD363
Plasmid#91829PurposeLoxP-flanked SEC donor for the SapTrap cloning systemDepositorInsertLoxP-SEC-LoxP
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJUMP22x-1A(sfGFP)
Plasmid#126969PurposeLevel 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Double UP mClover3 to mScarlet
Plasmid#120262PurposeNatively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.DepositorInsertmClover3/mScarletP2A-mcs
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7deltaE3
Plasmid#175748PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.DepositorInsertAdenovirus 5 genomic region 25043-35938
UseAdenoviral and Synthetic BiologyTagsExpressionMutationAdenovirus sequences 27859-30803 replaced with Ba…PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEC625
Plasmid#174368PurposeGolden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Kan)
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-15
Plasmid#119994PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SMAD2_HAe2_Bsd
Plasmid#167166PurposeBlasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.DepositorInsertRepair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
UseRepair template for gene editing in human cellsTagsblasticidinExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D-5
Plasmid#107865PurposeBacterial expression of PIS and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_hUmiRc2
Plasmid#25747PurposeEntry vector for cloning miR30-based shRNA driven by human U6 promoter.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP B56 gamma3 shRNA 14-1
Plasmid#10681DepositorInsertPP2A B56 gamma3 shRNA (PPP2R5C Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJUMP44-2A(sfGFP)
Plasmid#126988PurposeLevel 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK1200
Plasmid#84219PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherryDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry
Plasmid#107246PurposehM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.DepositorArticleInserthM3DREADD
UseAAVTagsABIcs, FLAG, and mCherryExpressionMutationT to D and S to D mutations in V2-tailPromoterhSYNAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-22
Plasmid#120001PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-mCh-Oct4i
Plasmid#21906DepositorInsertOct4i
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-11
Plasmid#119990PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS267
Plasmid#122380PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.N
Plasmid#79468PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, NeomycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only