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zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

Plasmid Kit

#1000000171

Purpose

Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

Depositor

Johannes Stuttmann, Sylvestre Marillonnet

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne-

Bacterial Strain

#182777

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- endA-

Bacterial Strain

#182774

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- lon-

Bacterial Strain

#182773

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne- lon-

Bacterial Strain

#182778

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- endA- lon-

Bacterial Strain

#182775

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
pCMV Flag WT-PTEN

Plasmid

#22231

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Sep 9. ():.

Insert

PTEN (PTEN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV Flag dPDZ-PTEN (1-400)

Plasmid

#22232

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Sep 9. ():.

Insert

PTEN (Pten Mouse)

Use

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

PDZ binding domain deleted. Contains amino acids …

Promoter

Available since

March 19, 2010

Availability

Academic Institutions and Nonprofits only
pMDC32-HPB

Plasmid

#32078

Depositor

Fumi Katagiri

Article

Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

Type

Empty backbone

Use

Gateway destination binary vector

Tags

HA-Prescission-Biotin

Expression

Mutation

Promoter

35S promoter

Available since

Dec. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC-pRPS2-HPB

Plasmid

#32077

Depositor

Fumi Katagiri

Article

Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

Type

Empty backbone

Use

Gateway destination binary vector

Tags

HA-Prescission-Biotin

Expression

Mutation

Promoter

RPS2 promoter

Available since

Dec. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAL-SK-swi3+

Plasmid

#15982

Depositor

Eishi Noguchi

Article

Noguchi et al Mol Cell Biol. 2004 Oct . 24(19):8342-55

Insert

Swi3 (swi3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only
RFP-zyxin

Plasmid

#26720

Depositor

Anna Huttenlocher

Article

Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

Insert

Zyxin (ZYX Human, unknown)

Use

Tags

RFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX-Baf155

Plasmid

#25856

Depositor

Hans Schöler

Article

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brg1 associated factor 155 (Smarcc1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
FKB

Plasmid

#12499

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

FKB

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pHIS2-hsSTAM1 (1-143)

Plasmid

#21498

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

phis2- STAM1(1-143) (STAM Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
PL2

Plasmid

#12395

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

PL2

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
PL1

Plasmid

#12394

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

PL1

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
LMB

Plasmid

#12502

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

LMB

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
ACY

Plasmid

#12396

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

ACY

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
TEN

Plasmid

#12498

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

TEN

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

Showing: 801 - 820 of 20036 results

Results per page:

Sort results by:

zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

C321.∆A T7RNAP ompT- rne-

C321.∆A ompT- rne- endA-

C321.∆A ompT- rne- lon-

C321.∆A T7RNAP ompT- rne- lon-

C321.∆A ompT- rne- endA- lon-

pCMV Flag WT-PTEN

pCMV Flag dPDZ-PTEN (1-400)

pMDC32-HPB

pMDC-pRPS2-HPB

pAL-SK-swi3+

RFP-zyxin

pMX-Baf155

FKB

pHIS2-hsSTAM1 (1-143)

PL2

PL1

LMB

ACY

TEN

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