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Plasmid#209232PurposeMammalian expression of ATP10ADepositorInsertATP10A (ATP10A Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP8B3-HA
Plasmid#209228PurposeMammalian expression of ATP8B3DepositorInsertATP8B3 (ATP8B3 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-C1-crRNA-cloning-EF1a-BA
Plasmid#204623PurposepiggyBac vector for crRNA cloning and expression. Blasticidin selectable.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-DEST(r)
Plasmid#190238PurposeGateway destination vector, empty AAV vector backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)
Plasmid#158771PurposeExpression of synaptotagmin 1 variant with self-cleaving C2 domain.DepositorInsertSynaptotagmin 1 1-NS3/4a (Syt1 Mouse)
UseLentiviralTagsExpressionMutationFlexible linkers, ADVVCC/QMSY cleavage sitePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/MACROD1
Plasmid#172578PurposeFor generation of FlpIn lines with untagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-BirA(R118G)-HA-TARG1
Plasmid#172583PurposeFor transient expression of C-terminal BirA-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsBirA(R118G)-HAExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR/zeo-MACROD2
Plasmid#172573PurposeFor gateway cloning of N-terminal tagged MACROD2 constructs.DepositorInsertMACROD2 (MACROD2 Human)
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
UseTagsExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 313
Plasmid#98586PurposepNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).DepositorInsertcsrB
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCML 314
Plasmid#98584PurposepCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).DepositorInsertsCsrA
csrB
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-Mst2 SARAH
Plasmid#84298PurposeExpresses Mst2 SARAH domain for interaction and spectroscopy studies.DepositorInsertMst2 (STK3 Human)
UseTagsGSTExpressionBacterialMutationL443WPromotertacAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Cx43-7
Plasmid#57603PurposeLocalization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCx43
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET14b-PITPalpha N90F
Plasmid#58257Purposebacterial expression of human PITP alpha N90FDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationN90F (abolishes PI binding)PromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSETC PITPbeta N89F
Plasmid#58279Purposebacterial expression of PITP beta N89F mutantDepositorInsertPITP beta (PITPNB Human)
UseTagsHISExpressionBacterialMutationN89F (abolishes PI binding)PromoterT7Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha K61A
Plasmid#58258Purposebacterial expression of human PITP alpha K61ADepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-GST
Plasmid#41613DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-H122Y
Plasmid#33085DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationH122YPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only